Objective: To evaluate the capability of manganese (Mn(2+))-enhanced MRI (MEMRI) in a continuously semiquantitative assessment of rat optic nerve (ON) injury.
Methods: Forty rats were divided into three groups: (I) a control group that was submitted to MEMRI or to fluorescent labeling of retinal ganglion cells (RGCs) (n = 10); (II) an ON injury group that was submitted to MEMRI (n = 15); (III) an ON injury group that was submitted to fluorescent labeling of RGCs (n = 15). Groups II and III were examined at 3, 7, and 14 days post-lesion (dpl), when the contrast-to-noise ratio (CNR) of the retina and ON was measured on MEMRI images and the RGCs were counted by fluorescence microscopy and compared between the groups.
Results: In the control group, the intact visual pathway from the retina to the contralateral superior colliculus was visualized by MEMRI. In group II, continuous Mn(2+) enhancement was seen from the retina to the lesion site of the optic nerves at 3, 7, and 14 dpl. However, no Mn(2+) enhancement was observed distal to the lesion site at those time points. The observed Mn(2+) enhancement proximal to the ON lesion site declined between 7 and 14 dpl. The decrease in Mn(2+)-enhanced signal intensity at these sites at 7 and 14 dpl when compared to that at 3 dpl was significant (P < 0.05). The RGC density dropped by 6.84, 45.31, and 72.36 % at 3, 7, and 14 dpl, respectively.
Conclusion: MEMRI can be used to evaluate the structural changes after optic nerve injury.
Keywords: Fluoro-Gold; Magnetic resonance imaging; Manganese; Optic nerve injury.