MicroRNA (miR)-133a expression has been reported to be downregulated in numerous human malignancies. However, the expression levels and function of miR-133a have not yet been investigated in human glioma. In the present study, the expression of miR‑133a was analyzed by reverse transcription‑quantitative polymerase chain reaction. Following transfection of miR‑133a, cell proliferation, cell migration, cell invasion and luciferase assays, and western blot analysis were conducted in glioma cell lines. The present study demonstrated that miR‑133a was downregulated in human glioma tissues compared with in normal adjacent tissues. In addition, the results indicated that miR‑133a was likely to directly target matrix metallopeptidase 9 in glioma. These results suggest that miR-133a may be considered as a target for the treatment of human glioma.