Transforming growth factor-β1 induces cholesterol synthesis by increasing HMG-CoA reductase mRNA expression in keratinocytes

Takumi Yamane; Aimi Muramatsu; Mari Shimura; Kazuo Kobayashi-Hattori; Yuichi Oishi

doi:10.1080/09168451.2016.1153960

Transforming growth factor-β1 induces cholesterol synthesis by increasing HMG-CoA reductase mRNA expression in keratinocytes

Biosci Biotechnol Biochem. 2016 Jul;80(7):1379-81. doi: 10.1080/09168451.2016.1153960. Epub 2016 Mar 2.

Authors

Takumi Yamane¹, Aimi Muramatsu², Mari Shimura¹, Kazuo Kobayashi-Hattori², Yuichi Oishi¹

Affiliations

¹ a Faculty of Applied Bioscience, Department of Nutritional Science and Food Safety , Tokyo University of Agriculture , Tokyo , Japan.
² b Faculty of Applied Bioscience, Department of Nutritional Science , Tokyo University of Agriculture , Tokyo , Japan.

PMID: 26932266
DOI: 10.1080/09168451.2016.1153960

Abstract

In this study, we investigated the effect of TGF-β1 on cholesterol synthesis in human keratinocytes. TGF-β1 increased the level of cholesterol and the mRNA level of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase in human keratinocytes. These results show that TGF-β1 induces cholesterol synthesis by increasing HMG-CoA reductase mRNA expression in human keratinocytes.

Keywords: 3-hydroxy-3-methylglutaryl coenzyme A reductase; cholesterol; keratinocyte; transforming growth factor-β1.

MeSH terms

Acyl Coenzyme A / biosynthesis*
Cell Line
Cholesterol / agonists
Cholesterol / biosynthesis*
Gene Expression
Humans
Hydroxymethylglutaryl CoA Reductases / genetics*
Hydroxymethylglutaryl CoA Reductases / metabolism
Keratinocytes / cytology
Keratinocytes / drug effects
Keratinocytes / metabolism
RNA, Messenger / agonists
RNA, Messenger / genetics*
RNA, Messenger / metabolism
Transforming Growth Factor beta1 / pharmacology*

Substances

Acyl Coenzyme A
RNA, Messenger
Transforming Growth Factor beta1
3-hydroxy-3-methylglutaryl-coenzyme A
Cholesterol
Hydroxymethylglutaryl CoA Reductases