Photodynamic activity of thiophene-derived lysosome-specific dyes

Francesca Baldassarre; Federica Foglietta; Viviana Vergaro; Nadia Barbero; Agostina L Capodilupo; Loredana Serpe; Sonja Visentin; Antonio Tepore; Giuseppe Ciccarella

doi:10.1016/j.jphotobiol.2016.02.013

Photodynamic activity of thiophene-derived lysosome-specific dyes

J Photochem Photobiol B. 2016 May:158:16-22. doi: 10.1016/j.jphotobiol.2016.02.013. Epub 2016 Feb 16.

Authors

Francesca Baldassarre¹, Federica Foglietta², Viviana Vergaro¹, Nadia Barbero³, Agostina L Capodilupo⁴, Loredana Serpe², Sonja Visentin⁵, Antonio Tepore⁶, Giuseppe Ciccarella⁷

Affiliations

¹ University of Salento, Biological and Environmental Sciences Department, Via Monteroni, 73100 Lecce, Italy.
² University of Torino, Department of Drug Science and Technology, Via P. Giuria 9, 10125 Torino, Italy.
³ University of Torino, Department of Chemistry and NIS Interdepartmental Centre, Via P. Giuria 7, 10125 Torino, Italy.
⁴ Institute of Nanotechnology, CNR NANOTEC, Via Monteroni, 73100 Lecce, Italy.
⁵ University of Torino, Department of Molecular Biotechnology and Health Sciences, Department Innovation Center, Via Quarello 15B, 10135 Torino, Italy.
⁶ University of Salento, Department of Cultural Heritage, Via Monteroni, 73100 Lecce, Italy.
⁷ University of Salento, Biological and Environmental Sciences Department, Via Monteroni, 73100 Lecce, Italy; Institute of Nanotechnology, CNR NANOTEC, Via Monteroni, 73100 Lecce, Italy. Electronic address: giuseppe.ciccarella@unisalento.it.

PMID: 26930158
DOI: 10.1016/j.jphotobiol.2016.02.013

Abstract

The photodynamic activity occurring through the lysosome photo-damage is effective in terms of triggered synergic effects which can avoid chemo-resistance pathways. The potential photodynamic activity of two fluorescent lysosome-specific probes was studied providing their interaction with human serum albumin, demonstrating their in vitro generation of singlet oxygen and investigating the resulted photo-toxic effect in human cancer cells.

Keywords: Human serum albumin; Lysosomes; Photosensitizers; Reactive oxygen species; Singlet oxygen; Thiophene dyes.

MeSH terms

Cell Line, Tumor
Humans
Lysosomes / drug effects*
Photochemotherapy*
Radiation-Sensitizing Agents / pharmacology*
Thiophenes / pharmacology*

Substances

Radiation-Sensitizing Agents
Thiophenes