Overview of chemical imaging methods to address biological questions

Micron. 2016 May:84:23-36. doi: 10.1016/j.micron.2016.02.005. Epub 2016 Feb 11.

Abstract

Chemical imaging offers extensive possibilities for better understanding of biological systems by allowing the identification of chemical components at the tissue, cellular, and subcellular levels. In this review, we introduce modern methods for chemical imaging that can be applied to biological samples. This work is mainly addressed to the biological sciences community and includes the bases of different technologies, some examples of its application, as well as an introduction to approaches on combining multimodal data.

Keywords: EELS; EFTEM; NanoSIMS; PIXE; STEM; Synchrotron radiation micro-analysis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Elements*
  • Imaging, Three-Dimensional / methods
  • Microscopy, Electron / instrumentation
  • Microscopy, Electron / methods*
  • Microscopy, Electron, Scanning Transmission / methods
  • Molecular Imaging / methods*
  • Nanoparticles
  • Neurons / chemistry
  • Neurons / ultrastructure

Substances

  • Elements