Recognition of a multiple antigen peptide containing sequence from mimotope of the dengue type 3 virus NS4B protein by human antibodies

Nevis Amin; Maritza Pupo; Alicia Aguilar; Susana Vázquez; Yamira Caballero; Rolando Ochoa; María G Guzmán; Armando Acosta

doi:10.1016/j.apjtm.2016.01.019

Recognition of a multiple antigen peptide containing sequence from mimotope of the dengue type 3 virus NS4B protein by human antibodies

Asian Pac J Trop Med. 2016 Feb;9(2):130-3. doi: 10.1016/j.apjtm.2016.01.019. Epub 2016 Jan 11.

Authors

Nevis Amin¹, Maritza Pupo², Alicia Aguilar³, Susana Vázquez², Yamira Caballero², Rolando Ochoa³, María G Guzmán², Armando Acosta³

Affiliations

¹ Implementation and Design of Clinical Trials, Clinical Research and Impact Evaluation Direction, Finlay Institute, 11600, Havana, Cuba. Electronic address: namin@finlay.edu.cu.
² 'Pedro Kourí' Tropical Medicine Institute (IPK), PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, 17100, Havana, Cuba.
³ Implementation and Design of Clinical Trials, Clinical Research and Impact Evaluation Direction, Finlay Institute, 11600, Havana, Cuba.

PMID: 26919941
DOI: 10.1016/j.apjtm.2016.01.019

Abstract

Objective: To evaluate the recognition of NS4B mimotope, as multiple antigen peptide (MAP), by dengue antibodies presents in serum samples from patients with different serotype infections.

Methods: A MAP containing mimotope sequence was synthesized and used to evaluate the recognition of NS4B mimotope as MAP by a panel of 66 human sera from dengue cases by an indirect ELISA assay.

Results: The MAP differentiated between sera from dengue viruses infected patients and sera from healthy individuals and the best reactivity was shown by serum from dengue type 3 virus patients. The recognition was more intense with serum from patients with secondary infection.

Conclusions: The findings suggest the potential use of NS4B mimotope on the development of a multi-epitope diagnostic tool. These results are important for further immunogenicity studies.

Keywords: Antibodies; Dengue virus; MAP; NS4B.