Dengue virus non-structural 1 protein interacts with heterogeneous nuclear ribonucleoprotein H in human monocytic cells

Drishya Diwaker; K P Mishra; L Ganju; S B Singh

doi:10.1016/j.apjtm.2016.01.015

Dengue virus non-structural 1 protein interacts with heterogeneous nuclear ribonucleoprotein H in human monocytic cells

Asian Pac J Trop Med. 2016 Feb;9(2):112-8. doi: 10.1016/j.apjtm.2016.01.015. Epub 2016 Jan 11.

Authors

Drishya Diwaker¹, K P Mishra², L Ganju¹, S B Singh¹

Affiliations

¹ Immunomodulation Lab, Defence Institute of Physiology & Allied Sciences, Delhi, India.
² Immunomodulation Lab, Defence Institute of Physiology & Allied Sciences, Delhi, India. Electronic address: kpmpgi@rediffmail.com.

PMID: 26919938
DOI: 10.1016/j.apjtm.2016.01.015

Abstract

Objective: To study protein-protein interaction between heterogeneous nuclear ribonucleoprotein H (hnRNP H) and Dengue virus (DENV) proteins.

Methods: DENV proteins were screened against the host hnRNP H protein, in order to identify the host-viral protein-protein interactions in DENV infected THP-1 cells by co-immunoprecipitation. The co-localization of the interacting proteins was further confirmed by immunofluorescence microscopy.

Results: The host protein hnRNP H was found to interact with DENV non-structural 1 protein and help the virus to multiply in the cell.

Conclusions: The non-structural 1 glycoprotein is a key modulator of host immune response and is also involved in viral replication. Therefore, disruption of this key interaction between hnRNP H and DENV non-structural 1 could be an important therapeutic strategy for management of DENV infection.

Keywords: Dengue virus; Heterogeneous nuclear ribonucleoprotein H; Non-structural 1 protein; Protein interactions.