Developing a biohybrid lung - sufficient endothelialization of poly-4-methly-1-pentene gas exchange hollow-fiber membranes

Bettina Wiegmann; Heide von Seggern; Klaus Höffler; Sotirios Korossis; Daniele Dipresa; Michael Pflaum; Sabrina Schmeckebier; Jörg Seume; Axel Haverich

doi:10.1016/j.jmbbm.2016.01.032

Developing a biohybrid lung - sufficient endothelialization of poly-4-methly-1-pentene gas exchange hollow-fiber membranes

J Mech Behav Biomed Mater. 2016 Jul:60:301-311. doi: 10.1016/j.jmbbm.2016.01.032. Epub 2016 Feb 11.

Authors

Bettina Wiegmann¹, Heide von Seggern², Klaus Höffler³, Sotirios Korossis⁴, Daniele Dipresa⁴, Michael Pflaum⁵, Sabrina Schmeckebier⁵, Jörg Seume², Axel Haverich⁶

Affiliations

¹ Department for Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany; Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department for Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Germany; Member of the German Centre for Lung Research (DZL), Hannover, Germany; REBIRTH-Cluster of Excellence, Hannover Medical School, Hannover, Germany. Electronic address: wiegmann.bettina@mh-hannover.de.
² Institute of Turbomachinery and Fluid Dynamics, Department of Mechanical Engineering, Leibniz University Hannover, Germany.
³ Department for Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany.
⁴ Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department for Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Germany.
⁵ Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department for Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Germany; REBIRTH-Cluster of Excellence, Hannover Medical School, Hannover, Germany.
⁶ Department for Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Hannover, Germany; Leibniz Research Laboratories for Biotechnology and Artificial Organs, Department for Cardiothoracic, Transplantation and Vascular Surgery, Hannover Medical School, Germany; Member of the German Centre for Lung Research (DZL), Hannover, Germany; REBIRTH-Cluster of Excellence, Hannover Medical School, Hannover, Germany.

PMID: 26919566
DOI: 10.1016/j.jmbbm.2016.01.032

Abstract

Working towards establishing a biohybrid lung with optimized hemocompatibility, this study analyzed the feasibility of establishing flow-resistant endothelium on heparin/albumin coated poly-4-methly-1-pentene hollow fiber gas exchange membranes (PMP-HFs). The seeding efficiency and proliferation of human cord blood derived endothelial cells (HCBEC) on PMP-HFs were analyzed under static conditions by WST-8 cell proliferation assay and fluorescence microscopy. The HCBEC monolayer integrity under different flow conditions was also assessed. Endothelial-specific phenotype verification, expression activation levels and thrombogenic state markers were quantified by real-time RT-PCR for cell-to-PMP-HF contact under static and dynamic conditions. The results demonstrated the feasibility of establishing a viable, confluent, and flow-resistant endothelial monolayer on the blood-contact surface of PMP-HFs, which maintained a physiological response to TNFα-stimulation and flow conditions. The endothelial phenotype, expression levels of adhesion molecules and thrombogenic state markers were unaffected by cell-to-PMP-HFs contact. These results represent a significant step towards establishing a biohybrid lung.

Keywords: Bioartificial Lung; Biohybrid lung; Endothelialization; Gas exchange membranes.

MeSH terms

Alkenes
Artificial Organs*
Cell Culture Techniques
Cells, Cultured
Endothelial Cells / cytology*
Humans
Lung*

Substances

Alkenes
1-pentene