Correlation between the Chemical and Genetic Relationships among Thymus saturejoides Genotypes Cultured under in vitro and in vivo Environments

Aicha Nordine; Sripada M Udupa; Driss Iraqi; Khalid Meksem; Mohamed Hmamouchi; Abdelmalek ElMeskaoui

doi:10.1002/cbdv.201500102

Correlation between the Chemical and Genetic Relationships among Thymus saturejoides Genotypes Cultured under in vitro and in vivo Environments

Chem Biodivers. 2016 Apr;13(4):387-94. doi: 10.1002/cbdv.201500102.

Authors

Aicha Nordine^{1

2}, Sripada M Udupa³, Driss Iraqi⁴, Khalid Meksem⁵, Mohamed Hmamouchi⁶, Abdelmalek ElMeskaoui^{1

2}

Affiliations

¹ Phytobiotechnology Unit, National Agency of Medicinal and Aromatic Plants, PB 159, Taounate, Morocco.
² University of Sidi Mohamed Ben Abdellah, Imouzzer Road, PB 2626, 30000, Fez, Morocco.
³ ICARDA-INRA Cooperative Research Project, International Center for Agricultural Research in the Dry Areas (ICARDA), PB 6299, Rabat, Morocco.
⁴ Biotechnology Research Unit, National Institute of Agronomic Research, PB 415, Rabat, Morocco.
⁵ Department of Plant, Soil & General Agriculture, Southern Illinois University, Carbondale, IL, 62901-6899, USA.
⁶ Faculty of Medicine and Pharmacy, Mohamed V University, Mohamed Belarbi El Alaoui ST, PB 6203, Rabat, Morocco.

PMID: 26919228
DOI: 10.1002/cbdv.201500102

Abstract

In this study, the in vitro and in vivo essential oil (EO) composition and genetic variability in six micropropagated genotypes of Thymus saturejoides Coss., a Mediterranean medicinal and aromatic plant, were analyzed by GC/MS and randomly amplified polymorphic DNA (RAPD). Yield and composition of the EO varied between genotypes. Cluster analysis based on RAPD data and EO grouped the six genotypes in three groups in both culture conditions, thus showing considerable intraspecific genetic and chemical variations. Applying the Mantel test, the result showed a significant correlation between the two proximity matrices RAPD and EO obtained from in vitro genotypes, whereas this correlation was not observed when using the EO obtained from the in vivo genotypes.

Keywords: Carvacrol; Essential oils; Randomly amplified polymorphic DNA; Thymol; Thymus saturejoides.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA, Plant / genetics
Gas Chromatography-Mass Spectrometry
Genotype*
In Vitro Techniques
Random Amplified Polymorphic DNA Technique
Thymus Plant / chemistry*
Thymus Plant / genetics

Substances

DNA, Plant