Flow cytometric detection of oxidative DNA damage in fish spermatozoa exposed to cadmium - Short communication

Szabolcs Nagy; Balázs Kakasi; Miklós Bercsényi

doi:10.1556/004.2016.013

Flow cytometric detection of oxidative DNA damage in fish spermatozoa exposed to cadmium - Short communication

Acta Vet Hung. 2016 Mar;64(1):120-4. doi: 10.1556/004.2016.013.

Authors

Szabolcs Nagy¹, Balázs Kakasi², Miklós Bercsényi¹

Affiliations

¹ Department of Animal Sciences and Animal Husbandry, Georgikon Faculty, University of Pannonia , Deák F. u. 16, H-8360 Keszthely , Hungary.
² Department of Limnology, Institute of Environmental Sciences, University of Pannonia , Veszprém , Hungary.

PMID: 26919149
DOI: 10.1556/004.2016.013

Abstract

The aim of the present pilot study was to apply a flow cytometric assay, the so-called OxyDNA test, to determine the level of oxidative DNA damage in fish spermatozoa exposed to different concentrations (0.01-10,000 mg/L) of cadmium. Milt was collected from three randomly selected Prussian carp (Carassius auratus gibelio) males. Oxidative DNA damage was assessed with the OxyDNA kit and using flow cytometry. The ratio of OxyDNA-positive events increased significantly at higher cadmium concentrations. The results indicate that direct contact of fish spermatozoa with cadmium-polluted water initiates genotoxic damage.

Keywords: Cadmium; DNA damage; OxyDNA; fish sperm; flow cytometry.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cadmium / pharmacology*
Cell Separation / methods
Cell Separation / veterinary
DNA Damage*
Dose-Response Relationship, Drug
Flow Cytometry / methods
Flow Cytometry / veterinary*
Male
Oxidative Stress
Pilot Projects
Semen / physiology
Spermatozoa / drug effects*
Spermatozoa / physiology

Substances

Cadmium