Interaction of Ca(2+)-dependent activator protein for secretion 1 (CAPS1) with septin family proteins in mouse brain

Neurosci Lett. 2016 Mar 23:617:232-5. doi: 10.1016/j.neulet.2016.02.035. Epub 2016 Feb 23.

Abstract

The Ca(2+)-dependent activator protein for secretion 1 (CAPS1) protein plays a regulatory role in the dense-core vesicle exocytosis pathway. To clarify the functions of this protein in the brain, we searched for novel interaction partners of CAPS1 by mass spectrometry. We identified a specific interaction of CAPS1 with septin family proteins. We also demonstrated that the C-terminal region of the CAPS1 protein binds to part of the deduced GTP-binding domain of septin proteins. It is possible that a tertiary complex of septin, CAPS, and syntaxin contributes to dense-core vesicle trafficking and exocytosis in neurons.

Keywords: Dense-core vesicle; Exocytosis; Immunoprecipitation; Mass spectrometry; Protein–protein interaction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / metabolism*
  • COS Cells
  • Calcium-Binding Proteins / metabolism*
  • Cell Line, Tumor
  • Chlorocebus aethiops
  • Mice
  • Nerve Tissue Proteins / metabolism*
  • Protein Binding
  • Protein Interaction Domains and Motifs
  • Septins / metabolism*

Substances

  • Cadps protein, mouse
  • Calcium-Binding Proteins
  • Nerve Tissue Proteins
  • Septins