Isolation and characterization of the Chrysanthemum nitrate transporter CmNRT1

Genet Mol Res. 2016 Jan 26;15(1). doi: 10.4238/gmr.15017148.

Abstract

In this study, the nitrate transporter gene CmNRT1 was isolated from the chrysanthemum variety 'Nannongxuefeng'. The full-length cDNA contains an open reading frame of 1761 bp encoding 587 residues. Using qRT-PCR, we found that CmNRT1 was induced by 10 mM NO3(-) in roots and shoots. Two Arabidopsis thaliana transgenic plants expressing CmNRT1 were selected for functional analyses. Root (15)N influx in wild-type and transgenic A. thaliana lines under 10 or 0.2 mM (15)NO3 was tested. Our results indicate that CmNRT1 encodes a constitutive component for a low-affinity transporter.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Anion Transport Proteins / chemistry
  • Anion Transport Proteins / genetics
  • Anion Transport Proteins / isolation & purification
  • Anion Transport Proteins / metabolism*
  • Arabidopsis / genetics
  • Chrysanthemum / metabolism*
  • Molecular Sequence Data
  • Nitrate Transporters
  • Phylogeny
  • Plant Proteins / chemistry
  • Plant Proteins / metabolism*
  • Plant Roots / metabolism*
  • Plants, Genetically Modified
  • Sequence Alignment

Substances

  • Anion Transport Proteins
  • Nitrate Transporters
  • Plant Proteins