One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle

Sci Rep. 2016 Feb 24:6:21953. doi: 10.1038/srep21953.

Abstract

Genetically modified cattle are important for developing new biomedical models and for an improved understanding of the pathophysiology of zoonotic diseases. However, genome editing and genetic engineering based on somatic cell nuclear transfer suffer from a low overall efficiency. Here, we established a highly efficient one-step multiplex gene transfer system into the bovine genome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified*
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Blastocyst / physiology
  • Cattle
  • DNA Transposable Elements*
  • Embryo Transfer / methods
  • Female
  • Fertilization in Vitro
  • Gene Expression
  • Gene Transfer Techniques
  • Genetic Engineering
  • Genome*
  • Luminescent Proteins / genetics*
  • Luminescent Proteins / metabolism
  • Male
  • Microinjections
  • Microscopy, Fluorescence
  • Oocytes / cytology
  • Oocytes / growth & development
  • Oocytes / metabolism
  • Plasmids / chemistry*
  • Plasmids / metabolism
  • Transgenes*
  • Transposases / genetics*
  • Transposases / metabolism

Substances

  • Bacterial Proteins
  • DNA Transposable Elements
  • Luminescent Proteins
  • yellow fluorescent protein, Bacteria
  • Transposases