18S rDNA dataset profiling microeukaryotic populations within Chicago area nearshore waters

Daniel Searle; Emily Sible; Alexandria Cooper; Catherine Putonti

doi:10.1016/j.dib.2015.12.042

18S rDNA dataset profiling microeukaryotic populations within Chicago area nearshore waters

Data Brief. 2016 Jan 7:6:526-9. doi: 10.1016/j.dib.2015.12.042. eCollection 2016 Mar.

Authors

Daniel Searle¹, Emily Sible², Alexandria Cooper², Catherine Putonti³

Affiliations

¹ The Woodhall School, 58 Harrison Lane, Behlehem, CT 06751, USA.
² Department of Biology, Loyola University Chicago, 1032 W Sheridan Rd, Chicago, IL 60660, USA.
³ Department of Biology, Loyola University Chicago, 1032 W Sheridan Rd, Chicago, IL 60660, USA; Bioinformatics Program, Loyola University Chicago, 1032 W Sheridan Rd, Chicago, IL 60660, USA; Department of Computer Science, Loyola University Chicago, 820 N Michigan Ave, Chicago, IL 60611, USA.

Abstract

Despite their critical role in the aquatic food web and nutrient cycling, microeukaryotes within freshwater environments are under-studied. Herein we present the first high-throughput molecular survey of microeukaryotes within Lake Michigan. Every two weeks from May 13 to August 5, 2014, we collected surface water samples from the nearshore waters of four Chicago area beaches: Gillson Park, Montrose Beach, 57th Street Beach, and Calumet Beach. Four biological replicates were collected for each sampling date and location, resulting in 112 samples. Eighty-nine of these samples were surveyed through targeted sequencing of the V7 and V8 regions of the 18S rDNA gene. Both technical and biological replicates were sequenced and are included in this dataset. Raw sequence data is available via NCBI's SRA database (BioProject PRJNA294919).

Keywords: Freshwater; Lake Michigan; Metagenomics; Microeukaryotic community.