Characterization of arginine kinase, anovel allergen of dermatophagoides farinae (Der f 20)

Peng Xing; Haiqiong Yu; Meng Li; Xiaojun Xiao; Congli Jiang; Lihua Mo; Min Zhang; Pingchang Yang; Zhigang Liu

Characterization of arginine kinase, anovel allergen of dermatophagoides farinae (Der f 20)

Am J Transl Res. 2015 Dec 15;7(12):2815-23. eCollection 2015.

Authors

Peng Xing¹, Haiqiong Yu², Meng Li², Xiaojun Xiao², Congli Jiang², Lihua Mo², Min Zhang³, Pingchang Yang¹, Zhigang Liu¹

Affiliations

¹ Otolaryngology Institutes, Allergy and Immunology Research Center, Shenzhen UniversityShenzhen 518060, China; The State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen University School of Medicine3688 Nanhai Blvd., Shenzhen 518060, China.
² The State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, Shenzhen University School of Medicine 3688 Nanhai Blvd., Shenzhen 518060, China.
³ First Affiliated Hospital of Shenzhen University, Shenzhen Second Peopl's Hospital Shenzhen, Guangdong 518035, China.

PMID: 26885278
PMCID: PMC4731678

Abstract

Objective: To characterize a novel allergen, the Dermatophagoides farinae-derived arginine kinase (Der f 20).

Methods: The protein of Der f 20 was synthesized by genetic engineering approaches. The allergenicity of Der f 20 was tested by enzyme-linked immunosorbent assay and an airway allergy mouse model.

Results: The Der f 20 gene was cloned andpresented in the Gene Bank with an accession number of AAP57094. The Der f 20 is an arginine kinase (AK), whichshowed a close relationship with D. pteronyssinus AK and Aleuroglyphusovatus AK. Western-blot and ELISA studies showed the IgE binding capacity of Der f 20 was 66.7% in the sera from 6 dust mite allergic patients. Immune inhibition assayresults showed the IgE cross-reactivity between Der f 20 and DME (Dust mite extract). Positive responses to Der f 20 were 41.2% as shown by skin prick tests in 17 DME-allergic patients. In vitro experimental results showed that Der f 20 induced Th2 cell differentiation and the expression of T cell Ig mucin domain molecule-4 (TIM4) in DCs. Conclusions; The Der f 20 protein is a novel subtype of thedust mite allergen.

Keywords: Arginine kinase; Der f 20; allergy; dermatophagoidesfarinae; prokaryotic expression.