Abstract
Bile acid plays an important role in regulating blood glucose, lipid and energy metabolism. The present study was implemented to determine the effect of duodenal-jejunal bypass (DJB) on FXR, TGR-5expression in terminal ileum and its bile acid-related mechanism on glucose and lipid metabolism. Immunohistochemistry was used to detect relative gene or protein expression in liver and intestine. Firstly, we found that expression of FXR in liver and terminal ileum of DJB group was significantly higher than that in S-DJB group (P<0.05). In addition, DJB dramatically increased the activation of TGR-5 in the liver of rats. Furthermore, PEPCK, G6Pase, FBPase 1 and GLP-1 were up-regulated by DJB. In conclusion, these results showed that bile acid ameliorated glucose and lipid metabolism through bile acid-FXR and bile acid- TGR-5 signaling pathway.
Keywords:
Duodenal-jejunal bypass; FXR; TGR-5; bile acid.
MeSH terms
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Anastomosis, Surgical
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Animals
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Bile Acids and Salts / metabolism*
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Diabetes Mellitus, Type 2 / metabolism
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Diabetes Mellitus, Type 2 / surgery*
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Disease Models, Animal
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Duodenum / surgery*
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Fructose-Bisphosphatase / metabolism
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Glucagon-Like Peptide 1 / metabolism
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Glucose / metabolism*
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Glucose-6-Phosphatase / metabolism
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Ileum / metabolism*
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Intracellular Signaling Peptides and Proteins / metabolism
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Jejunum / surgery*
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Lipid Metabolism*
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Liver / metabolism*
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Phosphoenolpyruvate Carboxykinase (GTP) / metabolism
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Rats, Sprague-Dawley
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Receptors, Cytoplasmic and Nuclear / metabolism
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Receptors, G-Protein-Coupled / metabolism
Substances
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Bile Acids and Salts
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Gpbar1 protein, rat
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Intracellular Signaling Peptides and Proteins
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Receptors, Cytoplasmic and Nuclear
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Receptors, G-Protein-Coupled
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farnesoid X-activated receptor
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Glucagon-Like Peptide 1
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Fructose-Bisphosphatase
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Glucose-6-Phosphatase
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Pck1 protein, rat
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Phosphoenolpyruvate Carboxykinase (GTP)
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Glucose