The use of real-time polymerase chain reaction with high resolution melting (real-time PCR-HRM) analysis for the detection and discrimination of nematodes Bursaphelenchus xylophilus and Bursaphelenchus mucronatus

Anna Filipiak; Beata Hasiów-Jaroszewska

doi:10.1016/j.mcp.2016.02.003

The use of real-time polymerase chain reaction with high resolution melting (real-time PCR-HRM) analysis for the detection and discrimination of nematodes Bursaphelenchus xylophilus and Bursaphelenchus mucronatus

Mol Cell Probes. 2016 Apr;30(2):113-7. doi: 10.1016/j.mcp.2016.02.003. Epub 2016 Feb 13.

Authors

Anna Filipiak¹, Beata Hasiów-Jaroszewska²

Affiliations

¹ Department of Biological Pest Control, Institute of Plant Protection, National Research Institute, Władysława Węgorka 20, 60-318, Poznan, Poland. Electronic address: A.Filipiak@iorpib.poznan.pl.
² Department of Virology and Bacteriology, Institute of Plant Protection, National Research Institute, Władysława Węgorka 20, 60-318, Poznan, Poland.

PMID: 26880540
DOI: 10.1016/j.mcp.2016.02.003

Abstract

The real-time PCR-HRM analysis was developed for the detection and discrimination of the quarantine nematode Bursaphelenchus xylophilus and Bursaphelenchus mucronatus. A set of primers was designed to target the ITS region of rDNA. The results have demonstrated that this analysis is a valuable tool for differentiation of these both species.

Keywords: Bursaphelenchus mucronatus; Bursaphelenchus xylophilus; Detection; Pine wilt disease; Quarantine nematode; Real-time PCR-HRM.

MeSH terms

Animals
DNA Primers / genetics
DNA, Ribosomal Spacer / genetics
Real-Time Polymerase Chain Reaction / methods*
Species Specificity
Tylenchida / cytology*
Tylenchida / genetics
Tylenchida / isolation & purification*

Substances

DNA Primers
DNA, Ribosomal Spacer