Reversible redox activity of ferrocene functionalized hydroxypropyl cellulose and its application to detect H2O2

Pingping Li; Hongliang Kang; Chao Zhang; Weiwei Li; Yong Huang; Ruigang Liu

doi:10.1016/j.carbpol.2015.11.077

Reversible redox activity of ferrocene functionalized hydroxypropyl cellulose and its application to detect H2O2

Carbohydr Polym. 2016 Apr 20:140:35-42. doi: 10.1016/j.carbpol.2015.11.077. Epub 2015 Dec 10.

Authors

Pingping Li¹, Hongliang Kang², Chao Zhang¹, Weiwei Li¹, Yong Huang³, Ruigang Liu⁴

Affiliations

¹ State Key Laboratory of Polymer Physics and Chemistry, Beijing National Laboratory for Molecular Sciences (BNLMS), Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 100049, China.
² State Key Laboratory of Polymer Physics and Chemistry, Beijing National Laboratory for Molecular Sciences (BNLMS), Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
³ State Key Laboratory of Polymer Physics and Chemistry, Beijing National Laboratory for Molecular Sciences (BNLMS), Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China; National Research Center for Engineering Plastics, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China. Electronic address: yhuang@iccas.ac.cn.
⁴ State Key Laboratory of Polymer Physics and Chemistry, Beijing National Laboratory for Molecular Sciences (BNLMS), Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China. Electronic address: rgliu@iccas.ac.cn.

PMID: 26876825
DOI: 10.1016/j.carbpol.2015.11.077

Abstract

Novel ferrocene functionalized hydroxypropyl cellulose (HPC-Fc) were prepared by azide-alkyne cycloaddition and characterized. HPC-Fc exhibits an excellent reversible redox activity and could establish amazing electron transfer ability between enzyme and electrode. HPC-Fc and horseradish peroxidase (HRP) were coated on a platinized carbon electrode to prepare an amperometric biosensor for hydrogen peroxide (H2O2) detection. The amperometric response was measured as a function of H2O2 concentration at a fixed potential of 0.35V in 100mM phosphate buffer solution (pH 7.0). The novel biosensor exhibits a fast linear response toward H2O2 in the range of 0.1-8μM with sensitivity of 4.21nA/μM. Moreover, the enzyme assays measured by the spectrophotometer method confirm that abundant hydroxyl groups of HPC backbones are conductive for HRP to maintaining or even enhancing their activity. The redox active HPC-Fc with the unique properties of both ferrocene and cellulose is a good candidate for biosensor applications.

Keywords: Biosensor; Ferrocene; Horseradish peroxidase; Hydroxypropyl cellulose; Redox activity.

Publication types

Research Support, Non-U.S. Gov't