Spheroid culture of LuCaP 136 patient-derived xenograft enables versatile preclinical models of prostate cancer

Clin Exp Metastasis. 2016 Apr;33(4):325-37. doi: 10.1007/s10585-016-9781-2. Epub 2016 Feb 12.

Abstract

LuCaP serially transplantable patient-derived xenografts (PDXs) are valuable preclinical models of locally advanced or metastatic prostate cancer. Using spheroid culture methodology, we recently established cell lines from several LuCaP PDXs. Here, we characterized in depth the features of xenografts derived from LuCaP 136 spheroid cultures and found faithful retention of the phenotype of the original PDX. In vitro culture enabled luciferase transfection into LuCaP 136 spheroids, facilitating in vivo imaging. We showed that LuCaP 136 spheroids formed intratibial, orthotopic, and subcutaneous tumors when re-introduced into mice. Intratibial tumors responded to castration and were highly osteosclerotic. LuCaP 136 is a realistic in vitro-in vivo preclinical model of a subtype of bone metastatic prostate cancer.

Keywords: Androgen-dependence; Bone metastasis; Osteosclerosis; Prostate cancer.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Neoplasms / pathology*
  • Bone Neoplasms / secondary
  • Cell Line, Tumor
  • Humans
  • Male
  • Mice
  • Neoplasm Metastasis*
  • Prostatic Neoplasms / pathology*
  • Spheroids, Cellular / pathology*
  • Xenograft Model Antitumor Assays