Human pegivirus detected in a patient with severe encephalitis using a metagenomic pan-virus array

Helena Fridholm; Line Østergaard Sørensen; Maiken W Rosenstierne; Henrik Nielsen; Finn Sellebjerg; Åse Bengård Andersen; Anders Fomsgaard

doi:10.1016/j.jcv.2016.01.013

Human pegivirus detected in a patient with severe encephalitis using a metagenomic pan-virus array

J Clin Virol. 2016 Apr:77:5-8. doi: 10.1016/j.jcv.2016.01.013. Epub 2016 Jan 29.

Authors

Helena Fridholm¹, Line Østergaard Sørensen², Maiken W Rosenstierne³, Henrik Nielsen², Finn Sellebjerg⁴, Åse Bengård Andersen⁵, Anders Fomsgaard⁶

Affiliations

¹ Virus Research and Development, Department of Microbiological Diagnostics and Virology, Statens Serum Institut, Copenhagen, Denmark. Electronic address: HEFR@ssi.dk.
² Department of Infectious Diseases, Aalborg University Hospital, Aalborg, Denmark.
³ Virus Research and Development, Department of Microbiological Diagnostics and Virology, Statens Serum Institut, Copenhagen, Denmark.
⁴ Department of Neurology, Rigshospitalet, University of Copenhagen, Copenhagen, Denmark.
⁵ Infectious Disease Research Unit, Clinical Institute, University of Southern Denmark, Denmark.
⁶ Virus Research and Development, Department of Microbiological Diagnostics and Virology, Statens Serum Institut, Copenhagen, Denmark; Infectious Disease Research Unit, Clinical Institute, University of Southern Denmark, Denmark.

Abstract

We have used a metagenomic microarray to detect genomic RNA from human pegivirus in serum and cerebrospinal fluid from a patient suffering from severe encephalitis. No other pathogen was detected. HPgV in cerebrospinal fluid during encephalitis has never been reported before and its prevalence in cerebrospinal fluid needs further investigation.

Keywords: Encephalitis; HPgV; Metagenomics; Microarray.

Publication types

Case Reports
Research Support, Non-U.S. Gov't
Review

MeSH terms

Adult
Encephalitis, Viral / diagnosis*
Encephalitis, Viral / virology*
Female
Flaviviridae / classification
Flaviviridae / genetics*
Humans
Metagenomics* / methods
Real-Time Polymerase Chain Reaction
Serologic Tests