Highly sensitive electrochemical biosensor based on nonlinear hybridization chain reaction for DNA detection

Liping Jia; Shanshan Shi; Rongna Ma; Wenli Jia; Huaisheng Wang

doi:10.1016/j.bios.2016.02.007

Highly sensitive electrochemical biosensor based on nonlinear hybridization chain reaction for DNA detection

Biosens Bioelectron. 2016 Jun 15:80:392-397. doi: 10.1016/j.bios.2016.02.007. Epub 2016 Feb 3.

Authors

Liping Jia¹, Shanshan Shi¹, Rongna Ma¹, Wenli Jia¹, Huaisheng Wang²

Affiliations

¹ Department of Chemistry, Liaocheng University, Liaocheng 252059, China.
² Department of Chemistry, Liaocheng University, Liaocheng 252059, China. Electronic address: hswang@lcu.edu.cn.

PMID: 26872213
DOI: 10.1016/j.bios.2016.02.007

Abstract

In the present work we demonstrated an ultrasensitive detection platform for specific DNA based on nonlinear hybridization chain reaction (HCR) by triggering chain-branching growth of DNA dendrimers. HCR was initiated by target DNA (tDNA) and finally formed dendritic structure by self-assembly. The electrochemical signal was drastically enhanced by capturing multiple catalytic peroxidase with high-ordered growth. Electrochemical signals were obtained by measuring the reduction current of oxidized 3, 3', 5, 5'-tetramethylbenzidine sulfate (TMB), which was generated by HRP in the presence of H2O2. This method exhibited ultrahigh sensitivity to tDNA with detection limit of 0.4 fM. Furthermore, the biosensor was also capable of discriminating single-nucleotide difference among concomitant DNA sequences.

Keywords: Biosensor; DNA; Nonlinear hybridization chain reaction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Benzidines / chemistry
Biosensing Techniques / methods
DNA / analysis*
Electrochemical Techniques / methods*
Hydrogen Peroxide / chemistry
Limit of Detection
Nucleic Acid Hybridization / methods*
Oxidation-Reduction

Substances

Benzidines
3,3',5,5'-tetramethylbenzidine
DNA
Hydrogen Peroxide