Proteome-wide dataset supporting the study of ancient metazoan macromolecular complexes

Sadhna Phanse; Cuihong Wan; Blake Borgeson; Fan Tu; Kevin Drew; Greg Clark; Xuejian Xiong; Olga Kagan; Julian Kwan; Alexandr Bezginov; Kyle Chessman; Swati Pal; Graham Cromar; Ophelia Papoulas; Zuyao Ni; Daniel R Boutz; Snejana Stoilova; Pierre C Havugimana; Xinghua Guo; Ramy H Malty; Mihail Sarov; Jack Greenblatt; Mohan Babu; W Brent Derry; Elisabeth R Tillier; John B Wallingford; John Parkinson; Edward M Marcotte; Andrew Emili

doi:10.1016/j.dib.2015.11.062

Proteome-wide dataset supporting the study of ancient metazoan macromolecular complexes

Data Brief. 2015 Dec 12:6:715-21. doi: 10.1016/j.dib.2015.11.062. eCollection 2016 Mar.

Authors

Sadhna Phanse¹, Cuihong Wan², Blake Borgeson¹, Fan Tu³, Kevin Drew³, Greg Clark⁴, Xuejian Xiong⁵, Olga Kagan¹, Julian Kwan⁶, Alexandr Bezginov⁴, Kyle Chessman⁵, Swati Pal⁵, Graham Cromar⁵, Ophelia Papoulas³, Zuyao Ni¹, Daniel R Boutz³, Snejana Stoilova¹, Pierre C Havugimana¹, Xinghua Guo¹, Ramy H Malty⁷, Mihail Sarov⁸, Jack Greenblatt⁶, Mohan Babu⁷, W Brent Derry⁵, Elisabeth R Tillier⁴, John B Wallingford⁹, John Parkinson⁵, Edward M Marcotte⁹, Andrew Emili⁶

Affiliations

¹ Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada.
² Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada; Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA.
³ Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA.
⁴ Department of Medical Biophysics, Toronto, Ontario, Canada.
⁵ Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada; Hospital for Sick Children, Toronto, Ontario, Canada.
⁶ Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario, Canada; Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.
⁷ Department of Biochemistry, University of Regina, Regina, Saskatchewan, Canada.
⁸ Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
⁹ Center for Systems and Synthetic Biology, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA; Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA.

Abstract

Our analysis examines the conservation of multiprotein complexes among metazoa through use of high resolution biochemical fractionation and precision mass spectrometry applied to soluble cell extracts from 5 representative model organisms Caenorhabditis elegans, Drosophila melanogaster, Mus musculus, Strongylocentrotus purpuratus, and Homo sapiens. The interaction network obtained from the data was validated globally in 4 distant species (Xenopus laevis, Nematostella vectensis, Dictyostelium discoideum, Saccharomyces cerevisiae) and locally by targeted affinity-purification experiments. Here we provide details of our massive set of supporting biochemical fractionation data available via ProteomeXchange (PXD002319-PXD002328), PPIs via BioGRID (185267); and interaction network projections via (http://metazoa.med.utoronto.ca) made fully accessible to allow further exploration. The datasets here are related to the research article on metazoan macromolecular complexes in Nature [1].

Keywords: Biochemical; Fractionation; Metazoa; Protein complexes; Proteomics.

Abstract

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