Accurate Extraction of Nanometer Distances in Multimers by Pulse EPR

Abstract

Pulse electron paramagnetic resonance (EPR) is gaining increasing importance in structural biology. The PELDOR (pulsed electron-electron double resonance) method allows extracting distance information on the nanometer scale. Here, we demonstrate the efficient extraction of distances from multimeric systems such as membrane-embedded ion channels where data analysis is commonly hindered by multi-spin effects.

Keywords: EPR spectroscopy; biomimetic synthesis; deer; ion channels; membrane proteins; structural biology.