Abstract
Natural products are an important source of modern medical development, e.g., antibiotics, anticancers, immune modulators, etc. and will continue to be a powerful driving force for the discovery of novel potential drugs. In the heterologous hosts, natural products are biosynthesized using dedicated metabolic networks. By gene engineering, pathway reconstructing, and enzyme engineering, metabolic networks can be modified to synthesize novel compounds containing enhanced structural feature or produce a large quantity of known valuable bioactive compounds. The review introduces some important technical platforms and relevant examples of genetic regulation and manipulation to improve natural product titers or drive novel secondary metabolite discoveries.
Keywords:
Discovery; Natural product; Regulation and manipulation.
Publication types
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Research Support, Non-U.S. Gov't
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Review
MeSH terms
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Anti-Bacterial Agents / biosynthesis
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Anti-Bacterial Agents / isolation & purification
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Antineoplastic Agents / isolation & purification
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Antineoplastic Agents / metabolism
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Aspergillus nidulans / genetics*
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Aspergillus nidulans / metabolism
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Biological Products / isolation & purification
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Biological Products / metabolism*
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Biosynthetic Pathways
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Drug Discovery*
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Escherichia coli / genetics*
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Escherichia coli / metabolism
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Gene Expression Regulation
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Genetic Engineering / methods*
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Glycopeptides / biosynthesis
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Glycopeptides / isolation & purification
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Humans
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Immunologic Factors / biosynthesis
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Immunologic Factors / isolation & purification
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Naphthoquinones / isolation & purification
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Naphthoquinones / metabolism
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Secondary Metabolism
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Streptomyces / genetics*
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Streptomyces / metabolism
Substances
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Anti-Bacterial Agents
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Antineoplastic Agents
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Biological Products
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Glycopeptides
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Immunologic Factors
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Naphthoquinones