HRT dependent performance and bacterial community population of granular hydrogen-producing mixed cultures fed with galactose

Gopalakrishnan Kumar; Periyasamy Sivagurunathan; Jeong-Hoon Park; Jong-Hun Park; Hee-Deung Park; Jeong-Jun Yoon; Sang-Hyoun Kim

doi:10.1016/j.biortech.2016.01.104

HRT dependent performance and bacterial community population of granular hydrogen-producing mixed cultures fed with galactose

Bioresour Technol. 2016 Apr:206:188-194. doi: 10.1016/j.biortech.2016.01.104. Epub 2016 Feb 2.

Authors

Gopalakrishnan Kumar¹, Periyasamy Sivagurunathan², Jeong-Hoon Park³, Jong-Hun Park⁴, Hee-Deung Park⁵, Jeong-Jun Yoon⁶, Sang-Hyoun Kim⁷

Affiliations

¹ Sustainable Management of Natural Resources and Environment Research Group, Faculty of Environment and Labour Safety, Ton Duc Thang University, Ho Chi Minh City, Viet Nam; Department of Environmental Engineering, Daegu University, Gyeongsan, Gyeongbuk 712-714, South Korea.
² Department of Environmental Engineering, Daegu University, Gyeongsan, Gyeongbuk 712-714, South Korea.
³ Civil, Environmental and Architectural Engineering, Korea University, Anam-Dong, Seongbuk-gu, Seoul 136-714, South Korea; IT Convergence Materials R&D Group, Korea Institute of Industrial Technology, Chungnam 330-825, South Korea.
⁴ Department of Environmental Engineering, Daegu University, Gyeongsan, Gyeongbuk 712-714, South Korea; Civil, Environmental and Architectural Engineering, Korea University, Anam-Dong, Seongbuk-gu, Seoul 136-714, South Korea.
⁵ Civil, Environmental and Architectural Engineering, Korea University, Anam-Dong, Seongbuk-gu, Seoul 136-714, South Korea.
⁶ IT Convergence Materials R&D Group, Korea Institute of Industrial Technology, Chungnam 330-825, South Korea.
⁷ Department of Environmental Engineering, Daegu University, Gyeongsan, Gyeongbuk 712-714, South Korea. Electronic address: sanghkim1@daegu.ac.kr.

PMID: 26859326
DOI: 10.1016/j.biortech.2016.01.104

Abstract

The effects of hydraulic retention times (HRTs-6, 3 and 2 h) on H2 production, operational stability and bacterial population response in a continuously stirred tank reactor (CSTR) were evaluated using galactose. A peak hydrogen production rate (HPR) of 25.9 L H2/L-d was obtained at a 3 h HRT with an organic loading rate (OLR) of 120 g/L-d, while the maximum hydrogen yield (HY) of 2.21 mol H2/mol galactose was obtained at a 6 h HRT (60 g galactose/L-d). Butyrate was dominant and the lactate concentration increased as HRT decreased, which significantly affected the HY. Biomass concentration (VSS) decreased from 16 to 3g/L at a 2 h HRT, leading to failure. A 3 h HRT supported the favorable growth of Clostridium species, as indicated by an increase in their populations from 25.4% to 27%, while significantly reducing Bacilli populations from 61.6% to 54.2%, indicating that this was the optimal condition.

Keywords: Butyrate; Galactose; Granular biomass; Hydraulic retention time; Lactate.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteria / drug effects
Bacteria / metabolism*
Bacteria / ultrastructure
Biofuels
Biomass
Bioreactors / microbiology
Clostridium / drug effects
Clostridium / growth & development
Fermentation / drug effects
Galactose / pharmacology*
Hydrogen / metabolism*
Phylogeny
Solubility
Time Factors

Substances

Biofuels
Hydrogen
Galactose