Glycopeptide-functionalized gold nanoparticles for antibody induction against the tumor associated mucin-1 glycoprotein

Bioorg Med Chem. 2016 Mar 1;24(5):1132-5. doi: 10.1016/j.bmc.2016.01.044. Epub 2016 Jan 22.

Abstract

We report the preparation of gold nanoparticle (AuNP)-based vaccine candidates against the tumor-associated form of the mucin-1 (MUC1) glycoprotein. Chimeric peptides, consisting of a glycopeptide sequence derived from MUC1 and the T-cell epitope P30 sequence were immobilized on PEGylated AuNPs and the ability to induce selective antibodies in vivo was investigated. After immunization, mice showed significant MHC-II mediated immune responses and their antisera recognized human MCF-7 breast cancer cells. Nanoparticles designed according to this report may become key players in the development of anticancer vaccines.

Keywords: Antibodies; Cancer; Glycopeptides; Nanoparticles; Vaccines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cancer Vaccines / chemistry
  • Cancer Vaccines / immunology*
  • Epitopes, T-Lymphocyte / chemistry
  • Epitopes, T-Lymphocyte / immunology*
  • Genes, MHC Class II
  • Glycopeptides / chemistry
  • Glycopeptides / immunology*
  • Gold / chemistry*
  • Humans
  • Immunization
  • MCF-7 Cells
  • Metal Nanoparticles / chemistry*
  • Mice
  • Molecular Sequence Data
  • Mucin-1 / chemistry
  • Mucin-1 / immunology*
  • Neoplasms / immunology
  • Neoplasms / prevention & control

Substances

  • Cancer Vaccines
  • Epitopes, T-Lymphocyte
  • Glycopeptides
  • Mucin-1
  • Gold