Abstract
Intrinsically disordered proteins (IDPs) are characterized by a lack of persistent structure. Since their identification more than a decade ago, many questions regarding their functional relevance and interaction mechanisms remain unanswered. Although most experiments have taken equilibrium and structural perspectives, fewer studies have investigated the kinetics of their interactions. Here we review and highlight the type of information that can be gained from kinetic studies. In particular, we show how kinetic studies of coupled folding and binding reactions, an important class of signaling event, are needed to determine mechanisms.
Keywords:
IDP; biophysics; coupled folding and binding; electrostatics; kinetics; phi-value; protein dynamic; protein electrostatics; protein folding; protein-protein interactions; residual structure; signaling.
© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.
Publication types
-
Research Support, Non-U.S. Gov't
-
Review
MeSH terms
-
Apoptosis Regulatory Proteins / chemistry*
-
Apoptosis Regulatory Proteins / genetics
-
Apoptosis Regulatory Proteins / metabolism
-
CREB-Binding Protein / chemistry*
-
CREB-Binding Protein / genetics
-
CREB-Binding Protein / metabolism
-
Cyclic AMP Response Element-Binding Protein / chemistry*
-
Cyclic AMP Response Element-Binding Protein / genetics
-
Cyclic AMP Response Element-Binding Protein / metabolism
-
Humans
-
Hydrophobic and Hydrophilic Interactions
-
Intrinsically Disordered Proteins / chemistry*
-
Intrinsically Disordered Proteins / genetics
-
Intrinsically Disordered Proteins / metabolism
-
Kinetics
-
Molecular Dynamics Simulation
-
Myeloid Cell Leukemia Sequence 1 Protein / chemistry*
-
Myeloid Cell Leukemia Sequence 1 Protein / genetics
-
Myeloid Cell Leukemia Sequence 1 Protein / metabolism
-
Protein Binding
-
Protein Folding
-
Protein Interaction Domains and Motifs
-
Proto-Oncogene Proteins / chemistry*
-
Proto-Oncogene Proteins / genetics
-
Proto-Oncogene Proteins / metabolism
-
Signal Transduction
-
Static Electricity
-
Thermodynamics
Substances
-
Apoptosis Regulatory Proteins
-
BBC3 protein, human
-
CREB1 protein, human
-
Cyclic AMP Response Element-Binding Protein
-
Intrinsically Disordered Proteins
-
MCL1 protein, human
-
Myeloid Cell Leukemia Sequence 1 Protein
-
Proto-Oncogene Proteins
-
CREB-Binding Protein
-
CREBBP protein, human
Associated data
-
PDB/1FEV
-
PDB/1KBH
-
PDB/1SBO
-
PDB/2ROC
-
PDB/3LBX