Single-Molecule Encapsulation: A Straightforward Route to Highly Stable and Printable Enzymes

Ana Beloqui; Sarah Baur; Vanessa Trouillet; Alexander Welle; Jeppe Madsen; Martin Bastmeyer; Guillaume Delaittre

doi:10.1002/smll.201503405

Single-Molecule Encapsulation: A Straightforward Route to Highly Stable and Printable Enzymes

Small. 2016 Apr 6;12(13):1716-22. doi: 10.1002/smll.201503405. Epub 2016 Feb 5.

Authors

Ana Beloqui^{1

2}, Sarah Baur³, Vanessa Trouillet^{4

5}, Alexander Welle^{2

5

6}, Jeppe Madsen⁷, Martin Bastmeyer^{3

8}, Guillaume Delaittre^{1

2}

Affiliations

¹ Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
² Preparative Macromolecular Chemistry, Institute for Technical Chemistry and Polymer Chemistry, Karlsruhe Institute of Technology (KIT), Engesserstrasse 15, 76131, Karlsruhe, Germany.
³ Zoological Institute, Department of Cell and Neurobiology, Karlsruhe Institute of Technology (KIT), Haid-und-Neu-Strasse 9, 76131, Karlsruhe, Germany.
⁴ Institute for Applied Materials (IAM), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
⁵ Karlsruhe Nano Micro Facility (KNMF), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
⁶ Institute for Biological Interfaces (IBG), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
⁷ Department of Chemistry, University of Sheffield, Brook Hill, Sheffield, S3 7HF, UK.
⁸ Institute for Functional Interfaces (IFG), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.

PMID: 26849308
DOI: 10.1002/smll.201503405

Abstract

A mild, fast, and sequence-independent method for controlled enzyme immobilization is presented. This novel approach involves the encapsulation of single-enzyme molecules and the covalent attachment of these nanobiocatalysts onto surfaces. Fast and mild immobilization conditions, combined with low nonspecific adsorption on hydrophobic substrates, enables well-defined surface patterns via microcontact printing. The biohybrid materials show enhanced activity in organic solvents.

Keywords: biocatalysis; encapsulation; immobilization; printable enzymes; thiol-ene.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adsorption
Biocatalysis
Enzyme Stability
Enzymes, Immobilized / metabolism*
Horseradish Peroxidase / metabolism*
Nanogels
Polyethylene Glycols / chemistry
Polyethyleneimine / chemistry
Printing / methods*

Substances

Enzymes, Immobilized
Nanogels
polyethylene glycol polyethyleneimine nanogel
Polyethylene Glycols
Polyethyleneimine
Horseradish Peroxidase