MicroRNA-96 Regulates Apoptosis by Targeting PDCD4 in Human Glioma Cells

Technol Cancer Res Treat. 2017 Feb;16(1):92-98. doi: 10.1177/1533034616629260. Epub 2016 Jul 7.

Abstract

Glioblastoma multiforme, the most common and aggressive form of primary brain tumor, presents a dismal prognosis. MicroRNAs play a critical role in the initiation, progression, and metastasis of cancer; however, the potential biological role of miRNAs in glioblastoma multiforme remains largely unknown. In our study, we found that microRNA-96 is upregulated in glioma tissues than in normal human brains. Transfection of microRNA-96 mimics into glioma cells significantly decreases apoptosis by suppressing PDCD4, a well-known tumor suppressor that is involved in apoptosis. In contrast, knockdown of microRNA-96 enhanced apoptosis. In vivo, microRNA-96 overexpression inhibits the apoptosis and increases tumor growth. These data suggest that microRNA-96 is a potential molecular target for glioma treatment.

Keywords: PDCD4; apoptosis; glioma; microRNA-96.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / genetics*
  • Apoptosis Regulatory Proteins / genetics*
  • Cell Line, Tumor
  • Disease Models, Animal
  • Gene Expression
  • Gene Expression Regulation, Neoplastic*
  • Genes, Tumor Suppressor
  • Glioma / genetics*
  • Humans
  • Mice
  • MicroRNAs / genetics*
  • RNA Interference*
  • RNA-Binding Proteins / genetics*
  • Xenograft Model Antitumor Assays

Substances

  • Apoptosis Regulatory Proteins
  • MIRN96 microRNA, human
  • MicroRNAs
  • PDCD4 protein, human
  • RNA-Binding Proteins