MWCNT-Fe3O4-based immuno-PCR for the early screening of nasopharyngeal carcinoma

Liu Chia-Ching; Sadhasivam Subramaniam; Savitha Sivasubramanian; Lin Feng-Huei

doi:10.1016/j.msec.2015.12.055

MWCNT-Fe3O4-based immuno-PCR for the early screening of nasopharyngeal carcinoma

Mater Sci Eng C Mater Biol Appl. 2016 Apr 1:61:422-8. doi: 10.1016/j.msec.2015.12.055. Epub 2015 Dec 31.

Authors

Liu Chia-Ching¹, Sadhasivam Subramaniam², Savitha Sivasubramanian³, Lin Feng-Huei⁴

Affiliations

¹ Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 100, Taiwan.
² Department of Microbial Biotechnology, Bharathiar University, Coimbatore 641 046, India.
³ Department of Biotechnology, Sree Sastha Institute of Engineering and Technology, Chennai, India.
⁴ Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei 100, Taiwan; Division of Biomedical Engineering and Nanomedicine Research, National Health Research Institutes, Miaoli 350, Taiwan. Electronic address: double@ntu.edu.tw.

PMID: 26838868
DOI: 10.1016/j.msec.2015.12.055

Abstract

Nasopharyngeal carcinoma (NPC) is the most prevalent form of malignancy in southeast China and its development is meticulously related to EBV pathogenesis. The current screening techniques are unsatisfactory in terms of the sensitivity and hence most of the NPC patients are diagnosed at an advanced stage. Herein, we report the multi-walled carbon nanotubes (MWCNTs) combined with iron oxide nanoparticles as a sensing surface for the early screening of nasopharyngeal carcinoma (NPC) by immuno-PCR (iPCR). The MWCNT-Fe3O4 nanocomposite was characterized by Fourier transform infrared spectra (FTIR), Raman spectra, X-ray diffraction (XRD) and high-resolution transmission electron microscopy (HR-TEM). The characterization techniques had confirmed the successful formation of MWCNT-Fe3O4 nanocomposites. The MWCNT-Fe3O4-based iPCR was effectively tested for the quantification of anti-EBV antibodies in human serum and the limit of detection (LOD) was compared with ELISA. The limit of detection by iPCR was valid until 1:10,000,000 fold dilution of NPC(+ve) human serum, whereas ELISA can detect the anti-EBV antibodies in human serum up to 1:100,000 fold dilution. The MWCNT-Fe3O4 offers an excellent surface area for the antigen-antibody binding and hence greater sensitivity was achieved.

Keywords: EBNA1 antigen; MWCNT; NPC; Nanocomposites; iPCR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Viral / blood*
Carcinoma
Enzyme-Linked Immunosorbent Assay
Epstein-Barr Virus Nuclear Antigens / chemistry
Epstein-Barr Virus Nuclear Antigens / immunology
Ferrosoferric Oxide / chemistry*
Humans
Immunoglobulin A / blood
Limit of Detection
Microscopy, Electron, Transmission
Nanocomposites / chemistry*
Nanotubes, Carbon / chemistry*
Nasopharyngeal Carcinoma
Nasopharyngeal Neoplasms / diagnosis*
Nasopharyngeal Neoplasms / virology
Polymerase Chain Reaction
Spectroscopy, Fourier Transform Infrared

Substances

Antibodies, Viral
Epstein-Barr Virus Nuclear Antigens
Immunoglobulin A
Nanotubes, Carbon
EBV-encoded nuclear antigen 1
Ferrosoferric Oxide