Graphene oxide for rapid determination of testosterone in the presence of cetyltrimethylammonium bromide in urine and blood plasma of athletes

Rashid Heidarimoghadam; Omid Akhavan; Elham Ghaderi; Ehsan Hashemi; Seyede Shima Mortazavi; Abbas Farmany

doi:10.1016/j.msec.2015.12.005

Graphene oxide for rapid determination of testosterone in the presence of cetyltrimethylammonium bromide in urine and blood plasma of athletes

Mater Sci Eng C Mater Biol Appl. 2016 Apr 1:61:246-50. doi: 10.1016/j.msec.2015.12.005. Epub 2015 Dec 4.

Authors

Rashid Heidarimoghadam¹, Omid Akhavan², Elham Ghaderi², Ehsan Hashemi³, Seyede Shima Mortazavi⁴, Abbas Farmany⁵

Affiliations

¹ Department of Ergonomics and Research Center for Health sciences, Hamedan University of Medical Sciences, Hamedan, Iran.
² Department of Physics, Sharif University of Technology, P.O. Box 11155-9161, Tehran, Iran; Institute for Nanoscience and Nanotechnology, Sharif University of Technology, P.O. Box 14588-89694, Tehran, Iran.
³ National Research Center for Transgenic Mouse & Animal Biotechnology Division, National Institute of Genetic Engineering and Biotechnology, P.O. Box 14965-161, Tehran, Iran.
⁴ Department of Chemistry, Hamedan Branch, Islamic Azad University, Hamedan, Iran.
⁵ Department of Chemistry, Hamedan Branch, Islamic Azad University, Hamedan, Iran. Electronic address: a.farmany@usa.com.

PMID: 26838847
DOI: 10.1016/j.msec.2015.12.005

Abstract

Electro-reduction behavior of testosterone at reduced graphene oxide/glassy carbon electrode (rGO/GCE) was studied. Cationic surfactant cetyltrimethylammonium bromide (CTAB) enhanced the reduction peak of testosterone. In borate buffer (pH5.4) CTAB-testosterone showed a reduction peak at -1.1 V (versus, Ag/AgCl). The increment of peak current obtained by deducting the reduction peak current of the CTAB-testosterone was rectilinear with testosterone concentration in the range of 2.0 to 210.0 nM, with a detection limit of 0.1 nM. The sensor was used for quantification of testosterone in biological fluids and drug.

Keywords: Biological fluids; Cetyltrimethylammonium bromide; Graphene; Testosterone.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Athletes*
Cetrimonium
Cetrimonium Compounds / chemistry*
Female
Graphite / chemistry*
Humans
Male
Sensitivity and Specificity
Testosterone / blood*
Testosterone / urine*

Substances

Cetrimonium Compounds
Testosterone
Graphite
Cetrimonium