Characterization and application of roxithromycin loaded cyclodextrin based nanoparticles for treatment of multidrug resistant bacteria

Farha Masood; Tariq Yasin; Habib Bukhari; Muhammad Mujahid

doi:10.1016/j.msec.2015.11.076

Characterization and application of roxithromycin loaded cyclodextrin based nanoparticles for treatment of multidrug resistant bacteria

Mater Sci Eng C Mater Biol Appl. 2016 Apr 1:61:1-7. doi: 10.1016/j.msec.2015.11.076. Epub 2015 Dec 2.

Authors

Farha Masood¹, Tariq Yasin², Habib Bukhari³, Muhammad Mujahid⁴

Affiliations

¹ Department of Biosciences, COMSATS Institute of Information Technology (CIIT), Islamabad, Pakistan. Electronic address: farha.masood@yahoo.com.
² Department of Metallurgy and Materials Engineering, Pakistan Institute of Engineering and Applied Sciences (PIEAS), Islamabad, Pakistan.
³ Department of Biosciences, COMSATS Institute of Information Technology (CIIT), Islamabad, Pakistan.
⁴ School of Chemicals & Materials Engineering (SCME), National University of Sciences and Technology (NUST), Islamabad, Pakistan.

PMID: 26838816
DOI: 10.1016/j.msec.2015.11.076

Abstract

An outbreak of infections with a high mortality rate caused by multidrug resistant (MDR) bacteria is one of the biggest health challenges globally. A class IV drug, roxithromycin (ROX), has poor solubility. In this study, ROX was first encapsulated in the cavity of each of the β-cyclodextrin (βCD) and hydroxypropyl-β-cyclodextrin (HPβCD). Then, each of the resulting βCD-ROX inclusion complex and HPβCD-ROX inclusion complex were separately loaded into poly-(lactic-co-glycolic acid) (PLGA) to synthesize βCD-ROX/PLGA and HPβCD-ROX/PLGA nanoparticles (NPs). Blank and ROX loaded PLGA (ROX-PLGA) NPs were also prepared. The loading efficiency of ROX is comparatively high for HPβCD-ROX/PLGA NPs in comparison to the βCD-ROX/PLGA NPs and ROX-PLGA NPs. All designed formulations showed significant (P<0.0001) antibacterial activity against the selected MDR bacterial strains. In a nutshell, this study demonstrated a great therapeutic potential of the above-mentioned delivery systems for treatment of MDR bacteria.

Keywords: Antimicrobial activity; Cyclodextrin; Inclusion complex; Nanoparticles; Poly-(lactic-co-glycolic acid); Roxithromycin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteria / growth & development*
Cyclodextrins* / chemistry
Cyclodextrins* / pharmacology
Drug Resistance, Multiple, Bacterial / drug effects*
Nanoparticles / chemistry*
Roxithromycin* / chemistry
Roxithromycin* / pharmacology

Substances

Cyclodextrins
Roxithromycin