Abstract
Protein aggregation is a common problem in protein biochemistry and is linked to many cellular pathologies and human diseases. The molecular chaperone ClpB can resolubilize and reactivate aggregated proteins. This unit describes the procedure for following reactivation of an aggregated enzyme glucose-6-phosphate dehydrogenase mediated by ClpB from Escherichia coli in cooperation with another molecular chaperone, DnaK. The procedures for purification of these chaperones are also described.
Keywords:
ClpB; DnaK; molecular chaperone; protein aggregation; protein misfolding.
Copyright © 2016 John Wiley & Sons, Inc.
Publication types
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Research Support, N.I.H., Extramural
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Review
MeSH terms
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Endopeptidase Clp
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Enzyme Activation
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Escherichia coli / chemistry*
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Escherichia coli Proteins / chemistry*
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism
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Glucosephosphate Dehydrogenase / chemistry*
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Glucosephosphate Dehydrogenase / genetics
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Glucosephosphate Dehydrogenase / metabolism
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HSP70 Heat-Shock Proteins / chemistry*
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HSP70 Heat-Shock Proteins / genetics
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HSP70 Heat-Shock Proteins / metabolism
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Heat-Shock Proteins / chemistry*
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Heat-Shock Proteins / genetics
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Heat-Shock Proteins / metabolism
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Humans
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Protein Aggregates*
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Solubility
Substances
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Escherichia coli Proteins
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HSP70 Heat-Shock Proteins
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Heat-Shock Proteins
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Protein Aggregates
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Glucosephosphate Dehydrogenase
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Endopeptidase Clp
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dnaK protein, E coli
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ClpB protein, E coli