Coupling Electrochemistry with Probe Electrospray Ionization Mass Spectrometry

Yi Cai; Pengyuan Liu; Michael A Held; Howard D Dewald; Hao Chen

doi:10.1002/cphc.201600033

Coupling Electrochemistry with Probe Electrospray Ionization Mass Spectrometry

Chemphyschem. 2016 Apr 18;17(8):1104-8. doi: 10.1002/cphc.201600033. Epub 2016 Feb 11.

Authors

Yi Cai¹, Pengyuan Liu¹, Michael A Held¹, Howard D Dewald¹, Hao Chen²

Affiliations

¹ Center for Intelligent Chemical Instrumentation, Department of Chemistry and Biochemistry, Edison Biotechnology Institute, Ohio University, Athens, OH, 45701, USA.
² Center for Intelligent Chemical Instrumentation, Department of Chemistry and Biochemistry, Edison Biotechnology Institute, Ohio University, Athens, OH, 45701, USA. chenh2@ohio.edu.

PMID: 26833903
DOI: 10.1002/cphc.201600033

Abstract

A new coupling of electrochemistry with mass spectrometry (MS) using probe electrospray ionization (PESI) is presented. Due to the high salt tolerance of PESI, the detection of electrochemical reaction products in room-temperature ionic liquids (RTILs) is realized for the first time. Furthermore, PESI-MS allows the analysis of electrochemical reaction products on different or multiple electrode surfaces. In addition, peptides and proteins fractionated through isoelectric focusing (IEF) in the presence of an external electric field can also be directly analyzed by using PESI-MS, suggesting a new and rapid characterization means for the IEF technique. This study reveals the versatility of EC/PESI-MS, which could have an impact in electrochemistry and bioanalysis fields.

Keywords: analytical methods; electrochemical reactions; ionic liquids; mass spectrometry; probe electrospray ionization.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Electrochemical Techniques*
Electrodes
Ionic Liquids / chemistry
Spectrometry, Mass, Electrospray Ionization
Surface Properties

Substances

Ionic Liquids