Adsorption of the cis-[Pt(NH3)2(P2O7)](2-) (phosphaplatin) on hydroxyapatite nanocrystals as a smart way to selectively release activated cis-[Pt(NH3)2Cl2] (cisplatin) in tumor tissues

Michele Benedetti; Federica De Castro; Alessandro Romano; Danilo Migoni; Barbara Piccinni; Tiziano Verri; Marco Lelli; Norberto Roveri; Francesco P Fanizzi

doi:10.1016/j.jinorgbio.2016.01.019

Adsorption of the cis-[Pt(NH3)2(P2O7)](2-) (phosphaplatin) on hydroxyapatite nanocrystals as a smart way to selectively release activated cis-[Pt(NH3)2Cl2] (cisplatin) in tumor tissues

J Inorg Biochem. 2016 Apr:157:73-9. doi: 10.1016/j.jinorgbio.2016.01.019. Epub 2016 Jan 20.

Authors

Michele Benedetti¹, Federica De Castro², Alessandro Romano³, Danilo Migoni², Barbara Piccinni², Tiziano Verri², Marco Lelli⁴, Norberto Roveri⁴, Francesco P Fanizzi⁵

Affiliations

¹ University of Salento, Department of Biological and Environmental Sciences and Technologies, Via Monteroni, 73100 Lecce, Italy. Electronic address: michele.benedetti@unisalento.it.
² University of Salento, Department of Biological and Environmental Sciences and Technologies, Via Monteroni, 73100 Lecce, Italy.
³ Neuropathology Unit, Institute of Experimental Neurology, Division of Neuroscience, IRCCS San Raffaele Scientific Institute, Via Olgettina 60, 20132 Milan, Italy.
⁴ Bioecoactive s.r.l., Via San Donato, 5 - 40057 Granarolo Dell'Emilia, Bologna, Italy.
⁵ University of Salento, Department of Biological and Environmental Sciences and Technologies, Via Monteroni, 73100 Lecce, Italy. Electronic address: fp.fanizzi@unisalento.it.

PMID: 26828286
DOI: 10.1016/j.jinorgbio.2016.01.019

Abstract

The relevant adsorption of cis-[Pt(NH3)2(P2O7)](2-) (phosphaplatin) on hydroxyapatite nanocrystals (nHAP) was observed and studied in water suspension. Phosphaplatin cytotoxicity, which is very low for HeLa, MCF-7 and HS-5 cell lines could be enhanced, reaching that of cisplatin, by interaction with solid nHAP. This effect stems from nHAP ability to catalyze the phosphaplatin hydrolysis, producing the same hydrolytic species responsible for cisplatin antitumor activity.

Keywords: Antitumor drug; Hydroxyapatite; Nanoparticles; Platinum drug; cisplatin; phosphaplatin.

MeSH terms

Adsorption
Antineoplastic Agents / chemistry*
Cisplatin / chemistry*
Durapatite / chemistry*
Microscopy, Electron, Transmission
Nanoparticles*

Substances

Antineoplastic Agents
Durapatite
Cisplatin