Genomic characterization of Pasteurella multocida HB01, a serotype A bovine isolate from China

Zhong Peng; Wan Liang; Wenjing Liu; Bin Wu; Biao Tang; Chen Tan; Rui Zhou; Huanchun Chen

doi:10.1016/j.gene.2016.01.041

Genomic characterization of Pasteurella multocida HB01, a serotype A bovine isolate from China

Gene. 2016 Apr 25;581(1):85-93. doi: 10.1016/j.gene.2016.01.041. Epub 2016 Jan 28.

Authors

Zhong Peng¹, Wan Liang², Wenjing Liu³, Bin Wu⁴, Biao Tang⁵, Chen Tan⁶, Rui Zhou⁷, Huanchun Chen⁸

Affiliations

¹ State Key Laboratory of Agricultural Microbiology, The Cooperative Innovation Center for Sustainable Pig Production, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: pengzhong525@163.com.
² State Key Laboratory of Agricultural Microbiology, The Cooperative Innovation Center for Sustainable Pig Production, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Education, The Cooperative Innovation Center for Sustainable Pig Production, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: wanleung828@webmail.hzau.edu.cn.
³ State Key Laboratory of Agricultural Microbiology, The Cooperative Innovation Center for Sustainable Pig Production, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: wenjing-918114@163.com.
⁴ State Key Laboratory of Agricultural Microbiology, The Cooperative Innovation Center for Sustainable Pig Production, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: wub@mail.hzau.edu.cn.
⁵ State Key Laboratory of Genetic Engineering, Department of Microbiology, School of Life Sciences, Fudan University, Shanghai 200000, China. Electronic address: tb_411@163.com.
⁶ State Key Laboratory of Agricultural Microbiology, The Cooperative Innovation Center for Sustainable Pig Production, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: tanchen@mail.hzau.edu.cn.
⁷ State Key Laboratory of Agricultural Microbiology, The Cooperative Innovation Center for Sustainable Pig Production, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: rzhou@mail.hzau.edu.cn.
⁸ State Key Laboratory of Agricultural Microbiology, The Cooperative Innovation Center for Sustainable Pig Production, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: chenhch@mail.hzau.edu.cn.

PMID: 26827796
DOI: 10.1016/j.gene.2016.01.041

Abstract

Pasteurella multocida infects various domestic and feral animals, generally causing clinical disease. To investigate P. multocida disease in cattle, we sequenced the complete genome of P. multocida HB01 (GenBank accession CP006976), a serotype A organism isolated from a cow in China. The genome is composed of a single circular chromosome of 2,416,068 base pairs containing 2212 protein-coding sequences, 6 ribosomal rRNA operons, and 56 tRNA genes. The present study confirms that P. multocida HB01 possesses a more complete metabolic pathway with an intact trichloroacetic acid cycle for anabolism compared with A. pleuropneumoniae and Haemophilus parasuis. This is the first time that this metabolic mechanism of P. multocida has been described. We also identified a full spectrum of genes related to known virulence factors of P. multocida. The differences in virulence factors between strains of different serotypes and origins were also compared. This comprehensive comparative genome analysis will help in further studies of the metabolic pathways, genetic basis of serotype, and virulence of P. multocida.

Keywords: Comparative analysis; Genome sequencing; Metabolism; Pasteurella multocida; Virulence factors.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacterial Adhesion
Cattle
China
Genome, Bacterial*
Lipopolysaccharides / metabolism
Pasteurella multocida / cytology
Pasteurella multocida / genetics*
Pasteurella multocida / isolation & purification
Pasteurella multocida / metabolism

Substances

Lipopolysaccharides