Characterization of SXT/R391 Integrative and Conjugative Elements in Proteus mirabilis Isolates from Food-Producing Animals in China

Chang-Wei Lei; An-Yun Zhang; Hong-Ning Wang; Bi-Hui Liu; Li-Qin Yang; Yong-Qiang Yang

doi:10.1128/AAC.02852-15

Characterization of SXT/R391 Integrative and Conjugative Elements in Proteus mirabilis Isolates from Food-Producing Animals in China

Antimicrob Agents Chemother. 2016 Jan 11;60(3):1935-8. doi: 10.1128/AAC.02852-15.

Authors

Chang-Wei Lei¹, An-Yun Zhang¹, Hong-Ning Wang², Bi-Hui Liu¹, Li-Qin Yang³, Yong-Qiang Yang¹

Affiliations

¹ College of Life Science, Sichuan University, Chengdu, People's Republic of China Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Chengdu, People's Republic of China Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, Chengdu, People's Republic of China 985 Project Science Innovative Platform for Resource and Environment Protection of Southwestern China, Chengdu, People's Republic of China.
² College of Life Science, Sichuan University, Chengdu, People's Republic of China Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, Chengdu, People's Republic of China Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, Chengdu, People's Republic of China 985 Project Science Innovative Platform for Resource and Environment Protection of Southwestern China, Chengdu, People's Republic of China whongning@163.com.
³ College of Life Science, Sichuan University, Chengdu, People's Republic of China Key Laboratory of Bio-resources and Eco-environment, Ministry of Education, Chengdu, People's Republic of China.

Abstract

SXT/R391 integrative and conjugative elements (ICEs) were detected in 8 out of 125 Proteus mirabilis isolates from food-producing animals in China. Whole-genome sequencing revealed that seven ICEs were identical to ICEPmiJpn1, carrying the cephalosporinase gene blaCMY-2. Another one, designated ICEPmiChn1, carried five resistance genes. All eight ICEs could be transferred to Escherichia coli via conjugation. The results highlight the idea that animal farms are important reservoir of the SXT/R391 ICE-containing P. mirabilis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacterial Proteins / genetics
Base Sequence
Chickens
China
Conjugation, Genetic / genetics
DNA Transposable Elements / genetics*
DNA, Bacterial / genetics
Drug Resistance, Multiple, Bacterial / genetics*
Escherichia coli / drug effects
Escherichia coli / genetics*
Farms
Genome
Microbial Sensitivity Tests
Poultry Diseases / microbiology
Proteus mirabilis / genetics*
Proteus mirabilis / isolation & purification*
Sequence Analysis, DNA
Swine
Swine Diseases / microbiology
beta-Lactamases / genetics*

Substances

Bacterial Proteins
DNA Transposable Elements
DNA, Bacterial
beta-lactamase CMY-2
beta-Lactamases

Grants and funding

This work was supported by “973” National Basic Research Program of China (project 2013CB127200), Earmarked Fund for Modern Agroindustry Technology Research System (grant CARS-41-K09), Special Fund for Agro-scientific Research in the Public Interest of China (grants 201303044 and 201403054), Science & Technology Pillar Program in Sichuan Province (grant 2013NZ0025), and the general program of National Natural Science Foundation of China (grants 31572547 and 31572548).