In vivo photothermal treatment by the peritumoral injection of macrophages loaded with gold nanoshells

Taeseok Daniel Yang; Wonshik Choi; Tai Hyun Yoon; Kyoung Jin Lee; Jae-Seung Lee; Jang Ho Joo; Min-Goo Lee; Hong Soon Yim; Kyung Min Choi; Byoungjae Kim; Jung Joo Lee; Heejin Kim; Doh Young Lee; Kwang-Yoon Jung; Seung-Kuk Baek

doi:10.1364/BOE.7.000185

In vivo photothermal treatment by the peritumoral injection of macrophages loaded with gold nanoshells

Biomed Opt Express. 2015 Dec 18;7(1):185-93. doi: 10.1364/BOE.7.000185. eCollection 2016 Jan 1.

Authors

Affiliations

¹ Korea University, Department of Physics, Seoul, South Korea.
² Korea University, Department of Materials Science and Engineering, Seoul, South Korea.
³ Korea University, Department of Physiology, Seoul, South Korea.
⁴ LivsMedInc., Seoul, South Korea.
⁵ Korea University, Department of Otolaryngology-Head and Neck Surgery, Seoul, South Korea.

Abstract

Photothermal treatment methods have been widely studied for their target specificity and potential for supplementing the limitations of conventional surgical treatments. In this study, we conducted in vivo photothermal treatments using macrophages containing nanoshells as live vectors. We injected macrophages at the peritumoral sites and observed that they had penetrated into the tumor approximately 48 hours after injection. Afterwards, we irradiated with a near-infrared laser for 2 minutes at 1 W/cm(2), causing cancer cell death. Our study identified the optimal conditions of the photothermal treatment and confirmed the feasibility of its use in in vivo treatments.

Keywords: (170.0170) Medical optics and biotechnology; (170.5180) Photodynamic therapy; (350.5340) Photothermal effects.