Draft Genome of Multidrug-Resistant Klebsiella pneumoniae 223/14 Carrying KPC-6, Isolated from a General Hospital in Malaysia

Norazah Ahmad; Teik Min Chong; Rohaidah Hashim; Surianti Shukor; Wai-Fong Yin; Kok-Gan Chan

doi:10.7150/jgen.13910

Draft Genome of Multidrug-Resistant Klebsiella pneumoniae 223/14 Carrying KPC-6, Isolated from a General Hospital in Malaysia

J Genomics. 2015 Nov 16:3:97-8. doi: 10.7150/jgen.13910. eCollection 2015.

Authors

Norazah Ahmad¹, Teik Min Chong², Rohaidah Hashim¹, Surianti Shukor¹, Wai-Fong Yin², Kok-Gan Chan²

Affiliations

¹ 1. Bacteriology Unit, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia.
² 2. Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia.

Abstract

We performed whole genome sequencing on a clinical multidrug-resistant Klebsiella pneumoniae strain 223/14. Investigation into its draft genome revealed the presence of KPC-6 variant, suggesting carbapenemase is present in this isolate. We found a plasmid-borne KPC gene (882 bp) inserted between two transposase genes in the genome of K. pneumoniae 223/14.

Keywords: Klebsiella pneumoniae Carbapenemase (KPC); multidrug resistance; whole genome sequencing.