Identification of Genes Relevant to Pesticides and Biology from Global Transcriptome Data of Monochamus alternatus Hope (Coleoptera: Cerambycidae) Larvae

Songqing Wu; Xiaoli Zhu; Zhaoxia Liu; Ensi Shao; Carballar-Lejarazú Rebeca; Yajie Guo; Yueting Xiong; Yani Mou; Runxue Xu; Xia Hu; Guanghong Liang; Shuangquan Zou; Xiong Guan; Feiping Zhang

doi:10.1371/journal.pone.0147855

Identification of Genes Relevant to Pesticides and Biology from Global Transcriptome Data of Monochamus alternatus Hope (Coleoptera: Cerambycidae) Larvae

PLoS One. 2016 Jan 27;11(1):e0147855. doi: 10.1371/journal.pone.0147855. eCollection 2016.

Authors

Songqing Wu^{1

2

3}, Xiaoli Zhu^{2

3}, Zhaoxia Liu^{2

3}, Ensi Shao^{2

3}, Carballar-Lejarazú Rebeca⁴, Yajie Guo¹, Yueting Xiong^{1

2}, Yani Mou^{1

2}, Runxue Xu², Xia Hu¹, Guanghong Liang¹, Shuangquan Zou¹, Xiong Guan^{2

3}, Feiping Zhang¹

Affiliations

¹ College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.
² Fujian-Taiwan Joint Center for Ecological Control of Crop Pests, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.
³ Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China.
⁴ Department of Molecular Biology and Biochemistry, University of California Irvine, Irvine, CA, 92697, United States of America.

Abstract

Monochamus alternatus Hope is the main vector in China of the Pine Wilt Disease caused by the pine wood nematode Bursaphelenchus xylophilus. Although chemical control is traditionally used to prevent pine wilt disease, new strategies based in biological control are promising ways for the management of the disease. However, there is no deep sequence analysis of Monochamus alternatus Hope that describes the transcriptome and no information is available about gene function of this insect vector. We used next generation sequencing technology to sequence the whole fourth instar larva transcriptome of Monochamus alternatus Hope and successfully built a Monochamus alternatus Hope transcriptome database. In total, 105,612 unigenes were assigned for Gene Ontology (GO) terms, information for 16,730 classified unigenes was obtained in the Clusters of Orthologous Groups (COGs) database, and 13,024 unigenes matched with 224 predicted pathways in the Kyoto Encyclopedia of Genes and Genome (KEGG). In addition, genes related to putative insecticide resistance-related genes, RNAi, the Bt receptor, intestinal digestive enzymes, possible future insect control targets and immune-related molecules are described. This study provides valuable basic information that can be used as a gateway to develop new molecular tools for Monochamus alternatus Hope control strategies.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Coleoptera / genetics*
High-Throughput Nucleotide Sequencing
Insect Proteins / genetics
Insect Vectors / genetics*
Insecticide Resistance
Larva / genetics
Pinus / parasitology*
Plant Diseases / etiology
Plant Diseases / parasitology*
Transcriptome*
Tylenchida / physiology*

Substances

Insect Proteins

Grants and funding

This study was supported by the Special Fund for Forestry Research in the Public Interest of China [Grant No. 201304401], Project of Fujian-Taiwan Joint Center for Ecological Control of Crop Pests [Minjiaoke (2013) 51], the United Fujian Provincial Health and Education Project for Tackling Key Research [Grant No. WKJ-FJ-25], National Natural Science Foundation of China [Grant No. 31301724, No. 31201574 and No. 31401802], Education Department Foundation of Fujian Province Distinguished Young Scholars in Universities of Fujian Province [Grant No.JA12092], Science Fund for Distinguished Young Scholars of Fujian Agriculture and Forestry University [Grant No. xjq201203], Key Projects of Fujian Provincial Department of Science and Technology [No. 2011|N5003 and No. 2013N0003], Natural Science Foundation of Fujian Province [Grant No. 2013J01079] and Programme for New Century Excellent Talents in Fujian Province [JA13089], the Special Fund for Grain Research in the Public Interest of China [Grant No. 201313002-3], the Special Fund for Key Construction Project of Fujian Agriculture and Forestry University [Grant No. 6112C035003], National Undergraduate Training Programme for Innovation and Entrepreneurship [Grant No. 201510389013, No. 201510389018, No. 201510389023, No. 201510389025, No. 201510389028, No. 201510389101 and No. 201510389173]. Science and Technology Project in Fujian Province Department of Education [JA15161], Central Finance Forestry Science and Technology Demonstration Project [Min (2015) TG017].