Nesterenkonia aurantiaca sp. nov., an alkaliphilic actinobacterium isolated from Antarctica

Ilaria Finore; Pierangelo Orlando; Paola Di Donato; Luigi Leone; Barbara Nicolaus; Annarita Poli

doi:10.1099/ijsem.0.000917

Nesterenkonia aurantiaca sp. nov., an alkaliphilic actinobacterium isolated from Antarctica

Int J Syst Evol Microbiol. 2016 Mar;66(3):1554-1560. doi: 10.1099/ijsem.0.000917. Epub 2016 Jan 26.

Authors

Ilaria Finore¹, Pierangelo Orlando², Paola Di Donato^{1

3}, Luigi Leone¹, Barbara Nicolaus¹, Annarita Poli¹

Affiliations

¹ Consiglio Nazionale delle Ricerche (C.N.R.), Istituto di Chimica Biomolecolare (I.C.B.), Via Campi Flegrei 34, 80078 Pozzuoli, Naples, Italy.
² Consiglio Nazionale delle Ricerche (C.N.R.), Istituto di Biochimica delle Proteine (I.B.P.), Via P. Castellino 111, 80124, Naples, Italy.
³ University of Naples 'Parthenope', Department of Sciences and Technologies, Centro Direzionale, Naples, Italy.

PMID: 26813578
DOI: 10.1099/ijsem.0.000917

Abstract

A Gram-stain-positive, non-endospore-forming, haloalkaliphilic actinobacterium, strain CK5^T, was isolated from a soil sample, collected at Cape King (Antarctica), and its taxonomic position was investigated by using a polyphasic approach. Cells were cocci with orange pigmentation, non-motile and grew optimally at 25 °C and pH 9.0-9.5 in the presence of 2 % (w/v) NaCl. Cellular membrane contained MK-7 (72 %) and MK-8 (28 %), and anteiso-C_{15 : 0} (64.8 %), iso-C_{16 : 0} (13.3 %), n-C_{17 : 0} (9.9 %), n-C_{16 : 0} (4.0 %), n-C_{14 : 0} (3.7 %) as major cellular fatty acids. The DNA G+C content was 64.8 mol%. Strain CK5^T, based on the 16S rRNA gene sequence similarity, was most closely related to Nesterenkonia jeotgali JG-241^T (99.5 %), Nesterenkonia sandarakina YIM 70009^T (99.4 %), Nesterenkonia lutea YIM 70081^T (99.4 %), Nesterenkonia halotolerans YIM 70084^T (99.3 %), Nesterenkonia xinjiangensis YIM 70097^T (97.2 %), Nesterenkonia flava CAAS 251^T (97.1 %) and Nesterekonia aethiopica CCUG 48939^T (97.1 %). Strain CK5^T revealed 31 % DNA-DNA relatedness with respect to N. sandarakina DSM 15664^T, 29 % with respect to N. jeotgali DSM 19081^T, 10 % with respect to N. lutea DSM 15666^T and 1 % with respect to N. halotolerans, DSM 15474^T, N. xinjiangensis DSM 15475^T, N. aethiopica DSM 17733^T and N. flava DSM 19422^T. On the basis of 16S rRNA gene sequences, DNA-DNA hybridization and chemotaxonomic characteristics, strain CK5^T represents a novel species of the genus Nesterenkonia, for which the name Nesterenkonia aurantiaca sp. nov. is proposed. The type strain is CK5^T ( = DSM 27373^T = JCM 19723^T).