During a vaccinia virus (VACV) outbreak in São Paulo State, Brazil, blood samples were collected from cows, humans, other domestic animals, and wild mammals. Samples from 3 dogs and 3 opossums were positive for VACV by PCR. Results of gene sequencing yielded major questions regarding other mammalian species acting as reservoirs of VACV.
Keywords: Brazil; PCR; São Paulo State; dogs; hosts; opossums; outbreak; reservoirs; vaccinia virus; viruses; zoonoses.