Effective Synthesis of Fluorescently Labeled Morpholino Nucleoside Triphosphate Derivatives

Nucleosides Nucleotides Nucleic Acids. 2016;35(1):32-42. doi: 10.1080/15257770.2015.1114125. Epub 2016 Jan 25.

Abstract

Morpholino nucleoside triphosphates (A, U, G, C, T) bearing the active functional amino group tethered to morpholine residue and their fluorescently labeled derivatives were synthesized. All compounds were characterized by (1)H, (13)C, and (31)P NMR, and mass spectrometry. A possibility of using fluorescently labeled morpholino nucleoside triphosphates as chain terminators in DNA sequencing is discussed.

Keywords: Morpholino nucleosides; fluorescent labeling; triphosphates.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fluorescent Dyes / chemistry*
  • Molecular Structure
  • Morpholines / chemistry*
  • Nucleotides / chemical synthesis*
  • Nucleotides / chemistry*

Substances

  • Fluorescent Dyes
  • Morpholines
  • Nucleotides