Abstract
The αββα metallo β-lactamase (MBL) fold (MBLf) was first observed in bacterial enzymes that catalyze the hydrolysis of almost all β-lactam antibiotics, but is now known to be widely distributed. The MBL core protein fold is present in human enzymes with diverse biological roles, including cell detoxification pathways and enabling resistance to clinically important anticancer medicines. Human (h)MBLf enzymes can bind metals, including zinc and iron ions, and catalyze a range of chemically interesting reactions, including both redox (e.g., ETHE1) and hydrolytic processes (e.g., Glyoxalase II, SNM1 nucleases, and CPSF73). With a view to promoting basic research on MBLf enzymes and their medicinal targeting, here we summarize current knowledge of the mechanisms and roles of these important molecules.
Keywords:
DNA repair; RNA processing; hydrogen sulphide metabolism; metallo β-lactamase fold protein; nuclease; β-lactam antibiotic and cancer drug resistance.
Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.
MeSH terms
-
Arabidopsis / enzymology
-
Arabidopsis / genetics
-
Bacteria / enzymology
-
Bacteria / genetics
-
DNA Repair Enzymes / chemistry*
-
DNA Repair Enzymes / genetics
-
DNA Repair Enzymes / metabolism
-
Exodeoxyribonucleases
-
Gene Expression
-
Humans
-
Hydrolysis
-
Mitochondrial Proteins / chemistry*
-
Mitochondrial Proteins / genetics
-
Mitochondrial Proteins / metabolism
-
Muscle Proteins / chemistry*
-
Muscle Proteins / genetics
-
Muscle Proteins / metabolism
-
Nuclear Proteins / chemistry*
-
Nuclear Proteins / genetics
-
Nuclear Proteins / metabolism
-
Nucleocytoplasmic Transport Proteins / chemistry*
-
Nucleocytoplasmic Transport Proteins / genetics
-
Nucleocytoplasmic Transport Proteins / metabolism
-
Protein Binding
-
Protein Conformation, alpha-Helical
-
Protein Conformation, beta-Strand
-
Protein Folding
-
Protein Interaction Domains and Motifs
-
Thiolester Hydrolases / chemistry*
-
Thiolester Hydrolases / genetics
-
Thiolester Hydrolases / metabolism
-
Zinc / chemistry*
-
Zinc / metabolism
-
beta-Lactamases / chemistry*
-
beta-Lactamases / genetics
-
beta-Lactamases / metabolism
-
beta-Lactams / chemistry
-
beta-Lactams / metabolism
Substances
-
ETHE1 protein, human
-
Mitochondrial Proteins
-
Muscle Proteins
-
Nuclear Proteins
-
Nucleocytoplasmic Transport Proteins
-
PNKD protein, human
-
beta-Lactams
-
DCLRE1B protein, human
-
Exodeoxyribonucleases
-
Thiolester Hydrolases
-
hydroxyacylglutathione hydrolase
-
beta-Lactamases
-
DNA Repair Enzymes
-
Zinc