Metagenomic analysis of a desulphurisation system used to treat biogas from vinasse methanisation

Marcela França Dias; Luis Felipe Colturato; João Paulo de Oliveira; Laura Rabelo Leite; Guilherme Oliveira; Carlos Augusto Chernicharo; Juliana Calabria de Araújo

doi:10.1016/j.biortech.2016.01.007

Metagenomic analysis of a desulphurisation system used to treat biogas from vinasse methanisation

Bioresour Technol. 2016 Apr:205:58-66. doi: 10.1016/j.biortech.2016.01.007. Epub 2016 Jan 8.

Authors

Marcela França Dias¹, Luis Felipe Colturato², João Paulo de Oliveira³, Laura Rabelo Leite⁴, Guilherme Oliveira⁴, Carlos Augusto Chernicharo¹, Juliana Calabria de Araújo⁵

Affiliations

¹ Department of Sanitary and Environmental Engineering, Federal University of Minas Gerais, Antonio Carlos Avenue, 6627, Belo Horizonte, Minas Gerais State 31270-90, Brazil.
² Department of Sanitary and Environmental Engineering, Federal University of Minas Gerais, Antonio Carlos Avenue, 6627, Belo Horizonte, Minas Gerais State 31270-90, Brazil; Methanum Waste and Energy, Alameda do Ingá, 840, Room 908, Vale do Sereno, Nova Lima, Minas Gerais State 34.000-000, Brazil.
³ Methanum Waste and Energy, Alameda do Ingá, 840, Room 908, Vale do Sereno, Nova Lima, Minas Gerais State 34.000-000, Brazil.
⁴ Genomics and Computational Biology Group, René Rachou Research Center, Oswaldo Cruz Foundation, Augusto de Lima Avenue, 1717, Belo Horizonte, Minas Gerais State 30.190-002, Brazil.
⁵ Department of Sanitary and Environmental Engineering, Federal University of Minas Gerais, Antonio Carlos Avenue, 6627, Belo Horizonte, Minas Gerais State 31270-90, Brazil. Electronic address: juliana@desa.ufmg.br.

PMID: 26803795
DOI: 10.1016/j.biortech.2016.01.007

Abstract

We investigated the response of microbial community to changes in H2S loading rate in a microaerated desulphurisation system treating biogas from vinasse methanisation. H2S removal efficiency was high, and both COD and DO seemed to be important parameters to biomass activity. DGGE analysis retrieved sequences of sulphide-oxidising bacteria (SOB), such as Thioalkalimicrobium sp. Deep sequencing analysis revealed that the microbial community was complex and remained constant throughout the experiment. Most sequences belonged to Firmicutes and Proteobacteria, and, to a lesser extent, Bacteroidetes, Chloroflexi, and Synergistetes. Despite the high sulphide removal efficiency, the abundance of the taxa of SOB was low, and was negatively affected by the high sulphide loading rate.

Keywords: Biogas; DGGE; Desulphurisation; Metagenomics; Vinasse.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteria
Biofuels*
Biomass
Bioreactors / microbiology*
Hydrogen Sulfide / chemistry
Metagenomics
Microbial Consortia*
Saccharum / metabolism*
Sulfides / metabolism*

Substances

Biofuels
Sulfides
Hydrogen Sulfide