А new Gal/GalNAc-specific lectin from the mussel Mytilus trossulus: Structure, tissue specificity, antimicrobial and antifungal activity

Irina V Chikalovets; Svetlana N Kovalchuk; Alina P Litovchenko; Valentina I Molchanova; Mikhail V Pivkin; Oleg V Chernikov

doi:10.1016/j.fsi.2016.01.020

А new Gal/GalNAc-specific lectin from the mussel Mytilus trossulus: Structure, tissue specificity, antimicrobial and antifungal activity

Fish Shellfish Immunol. 2016 Mar:50:27-33. doi: 10.1016/j.fsi.2016.01.020. Epub 2016 Jan 21.

Authors

Irina V Chikalovets¹, Svetlana N Kovalchuk², Alina P Litovchenko³, Valentina I Molchanova², Mikhail V Pivkin², Oleg V Chernikov²

Affiliations

¹ G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159, Prospect 100 let Vladivostoku, Vladivostok 690022, Russia; Far Eastern Federal University, 8, Sukhanova Str., Vladivostok 690950, Russia. Electronic address: ivchik6@mail.ru.
² G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159, Prospect 100 let Vladivostoku, Vladivostok 690022, Russia.
³ Far Eastern Federal University, 8, Sukhanova Str., Vladivostok 690950, Russia.

PMID: 26802895
DOI: 10.1016/j.fsi.2016.01.020

Abstract

In the present study, a new Gal/GalNAc specific lectin from the mussel Mytilus trossulus (designated as MTL) was identified, and its expression levels, both in tissues and toward pathogen stimulation, were then characterized. The MTL primary structure was determined via cDNA sequencing. Deduced sequence of 150 amino acid residues showed 89% similarity to lectins from the mussels Crenomytilus grayanus and Mytilus galloprovincialis that were the first members of a new family of zoolectins. The results indicated that the MTL might be involved in immune response toward pathogen infection, and it might perform different recognition specificity toward bacteria or fungi.

Keywords: Amino acid sequence; Antifungal and antibacterial activity; GalNAc/Gal-specific lectin; Innate immunity; Mollusc; Mytilus trossulus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Anti-Bacterial Agents / chemistry
Anti-Bacterial Agents / metabolism*
Antifungal Agents / chemistry
Antifungal Agents / metabolism*
Fungi / physiology
Lectins / chemistry
Lectins / genetics*
Lectins / metabolism
Mytilus / genetics*
Mytilus / immunology*
Mytilus / microbiology
Organ Specificity
Protein Structure, Secondary
Sequence Alignment
Vibrio / physiology

Substances

Anti-Bacterial Agents
Antifungal Agents
Lectins