A micro-controller unit (MCU) assisted immunoassay lab-on-a-chip is realized in 0.35 μm CMOS technology. The MCU automatically controls the detection procedure including blood filtration through a nonporous aluminum oxide membrane, bimolecular conjugation with antibodies attached to magnetic beads, electrolytic pumping, magnetic flushing and threshold detection based on Hall sensor array readout analysis. To verify the function of this chip, in-vitro Tumor necrosis factor- α (TNF-α) and N-terminal pro-brain natriuretic peptide (NT-proBNP) tests are performed by this 9 mm(2)-sized single chip. The cost, efficiency and portability are considerably improved compared to the prior art.