Immunoregulatory activities of polysaccharides from mung bean

Carbohydr Polym. 2016 Mar 30:139:61-6. doi: 10.1016/j.carbpol.2015.12.001. Epub 2015 Dec 11.

Abstract

Ultrasonic treatment was performed on water-extractable polysaccharides from the seed of mung beans. Purified by anion-exchange and gel filtration chromatography, MWP-1' and MWP-2' were obtained. Average molecular weights (Mws) of MWP-1' and MWP-2' were 68.4 kDa, and 52.4 kDa, respectively. Monosaccharides components analysis indicated that MWP-1' was composed of Rha, Ara, Man and Gal in a molar percent of 0.4:2.6:5.3:0.7. MWP-2' was composed of Ara, Man, Gal and Glc in a molar percent of 0.5:1.4:2.1:0.4. In vitro study showed that both polysaccharides samples were able to stimulate the production of secretory molecules (NO, TNF-α and IL-6) of RAW264.7 murine macrophages in a dosage dependent manner. MWP-2' seemed to be the most potent and induced significantly higher the NO production. These findings suggest that the ultrasonic treatment polysaccharides isolated in our study have immune potentiation effects on macrophages.

Keywords: Mung bean; Ultrasonic; Water-extractable polysaccharide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Fabaceae*
  • Immunologic Factors / chemistry
  • Immunologic Factors / isolation & purification
  • Immunologic Factors / pharmacology*
  • Interleukin-6 / metabolism
  • Macrophages / drug effects*
  • Macrophages / metabolism
  • Mice
  • Monosaccharides / analysis
  • Nitric Oxide / metabolism
  • Polysaccharides / chemistry
  • Polysaccharides / isolation & purification
  • Polysaccharides / pharmacology*
  • Tumor Necrosis Factor-alpha / metabolism
  • Ultrasonic Waves

Substances

  • Immunologic Factors
  • Interleukin-6
  • Monosaccharides
  • Polysaccharides
  • Tumor Necrosis Factor-alpha
  • interleukin-6, mouse
  • Nitric Oxide