Detection of dengue NS1 and NS3 proteins in placenta and umbilical cord in fetal and maternal death

J Med Virol. 2016 Aug;88(8):1448-52. doi: 10.1002/jmv.24479. Epub 2016 Feb 2.

Abstract

In Brazil, dengue is a public health problem with the occurrence of explosive epidemics. This study reports maternal and fetal deaths due to dengue and which tissues of placenta and umbilical cord were analyzed by molecular methods and immunohistochemistry. The dengue NS3 and NS1 detection revealed the viral presence in different cells from placenta and umbilical cord. In the latter, DENV-2 was detected at a viral titer of 1,02 × 10(4) amounts of viral RNA. It was shown that the DENV markers analyzed here may be an alternative approach for dengue fatal cases investigation, especially involving maternal and fetal death. J. Med. Virol. 88:1448-1452, 2016. © 2016 Wiley Periodicals, Inc.

Keywords: Brazil; NS1; NS3; dengue; maternal and fetal death.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Viral / immunology
  • Antigens, Viral / genetics
  • Brazil / epidemiology
  • Dengue / epidemiology
  • Dengue / virology*
  • Dengue Virus* / chemistry
  • Dengue Virus* / genetics
  • Dengue Virus* / immunology
  • Dengue Virus* / isolation & purification
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Fetal Death / etiology*
  • Humans
  • Immunohistochemistry
  • Macrophages / virology
  • Maternal Death / etiology*
  • Placenta / cytology
  • Placenta / pathology
  • Placenta / virology*
  • Pregnancy
  • RNA Helicases / genetics
  • RNA Helicases / immunology
  • RNA Helicases / isolation & purification
  • RNA, Viral / genetics
  • RNA, Viral / isolation & purification
  • Serine Endopeptidases / genetics
  • Serine Endopeptidases / immunology
  • Serine Endopeptidases / isolation & purification
  • Serologic Tests
  • Umbilical Cord / cytology
  • Umbilical Cord / pathology
  • Umbilical Cord / virology*
  • Viral Nonstructural Proteins / genetics
  • Viral Nonstructural Proteins / immunology
  • Viral Nonstructural Proteins / isolation & purification*
  • Young Adult

Substances

  • Antibodies, Viral
  • Antigens, Viral
  • NS1 protein, Dengue virus type 2
  • NS3 protein, flavivirus
  • RNA, Viral
  • Viral Nonstructural Proteins
  • Serine Endopeptidases
  • RNA Helicases