Imaging the role of toll-like receptor 4 on cell proliferation and inflammation after cerebral ischemia by positron emission tomography

J Cereb Blood Flow Metab. 2016 Apr;36(4):702-8. doi: 10.1177/0271678X15627657. Epub 2016 Jan 19.

Abstract

The influence of toll-like receptor 4 on neurogenesis and inflammation has been scarcely explored so far by using neuroimaging techniques. For this purpose, we performed magnetic resonance imaging and positron emission tomography with 3'-deoxy-3'-[(18)F]fluorothymidine and [(11)C]PK11195 at 2, 7, and 14 days following cerebral ischemia in TLR4(+/+)and TLR4(-/-)mice. MRI showed similar infarction volumes in both groups. Despite this, positron emission tomography with 3'-deoxy-3'-[(18)F]fluorothymidine and [(11)C]PK11195 evidenced an increase of neurogenesis and a decrease of inflammation in TLR4(-/-)mice after ischemia. These results evidence the versatility of neuroimaging techniques to monitor the role of toll-like receptor 4 after cerebral ischemia.

Keywords: 3′-deoxy-3′-[18F]fluorothymidine; Positron emission tomography; T2W-MRI; [11C]PK11195; cerebral ischemia; magnetic resonance imaging; middle cerebral artery occlusion; toll-like receptor 4.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain Ischemia / diagnostic imaging*
  • Brain Ischemia / genetics*
  • Cell Proliferation*
  • Cerebral Arterial Diseases / genetics
  • Cerebral Arterial Diseases / pathology
  • Cerebral Infarction / genetics
  • Cerebral Infarction / pathology
  • Dideoxynucleosides
  • Inflammation / genetics
  • Inflammation / metabolism*
  • Inflammation / pathology
  • Isoquinolines
  • Lateral Ventricles / pathology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Positron-Emission Tomography
  • Radiopharmaceuticals
  • Toll-Like Receptor 4 / genetics
  • Toll-Like Receptor 4 / metabolism*

Substances

  • Dideoxynucleosides
  • Isoquinolines
  • Radiopharmaceuticals
  • Tlr4 protein, mouse
  • Toll-Like Receptor 4
  • alovudine
  • PK 11195